Inflammation, Oxidative Stress and Atherosclerosis in Chronic Renal Failure Patients | ||||
| Bulletin of Egyptian Society for Physiological Sciences | ||||
| Article 6, Volume 31, Issue 1, December 2011, Page 75-91 PDF (719.76 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/besps.2011.35970 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Hosny Hassan* 1; Mona EL-Baz1; Mohammed Sobh2; Hossam Abo Zaid3; Taha Mostafa1 | ||||
| 1Medical Biochemistry Department, Faculty of Medicine. Assuit University | ||||
| 2Internal Medicine Department, Faculty of Medicine. Assuit University | ||||
| 3Radiology Department, Faculty of Medicine. Assuit University | ||||
| Abstract | ||||
| Background: Accelerated atherosclerosis is the major cause of mortality in patients on chronic hemodialysis (HD). Inflammation, increased oxidative stress and endothelial activation or dysfunction might be the major factors leading to high cardiovascular mortality rate in HD patients. Also, C667T mutation of methylenetetrahydrofolate reductase (MTHFR) might be associated with accelerated atherosclerosis. Aim: The present study was designed to clarify the role of inflammation, oxidative stress parameters, endothelial activation or dysfunction and genotyping of MTHFR enzyme which affect the level of homocysteine and their relation to carotid artery intima-media thickness (CIMT) as indicators of atherosclerosis. Subjects & Methods: 44 chronic hemodialysis (HD) patients and 40 healthy subjects were included in the study. Serum highly sensitive C reactive protein (hs-CRP) and IL-6 were measured as inflammatory markers, soluble vascular cell adhesion molecule-1 (sVCAM-1) was measured as a marker of endothelial activation and dysfunction. Serum thiobarbituric acid reactive substances (TBARS), total nitric oxide (NO), total peroxides (TP), total antioxidant capacity (TAC) and oxidative stress index (OSI) levels were determined as oxidative stress markers. Common carotid intima media thickness (CC-IMT) was assessed by carotid artery ultrasonography, genotyping of MTHFR enzyme which affect the level of homocysteine was analyzed by PCR –RFLIP technique. Results: Chronic HD patients had elevated levels of inflammatory markers (hs-CRP and IL-6), enhanced endothelial activation or dysfunction demonstrated by elevated VCAM-1 as compared by healthy controls. Also, they had enhanced oxidative stress indicated by the higher levels of NO, TBARS, TP, OSI and lower levels of TAC as compared to controls. Hemodialysis patients had significantly higher CC-IMT levels. There is a significant positive correlation between inflammatory cytokines (hs-CRP and IL-6), and each of TBARS, total NO, TP and OSI with CC-IMT Also, the previous parameters negatively correlated with TAC. There is no significant difference in the genotype of C667T MTHFR between patients and controls, but that mutation especially the TT genotype is associated with development of atherosclerosis as indicated by the increase of CCIMT. | ||||
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