Analysis of BRCA1 and BRCA2 Mutations in Eastern Egyptian Breast Cancer Patients | ||||
Bulletin of Egyptian Society for Physiological Sciences | ||||
Article 8, Volume 31, Issue 1, December 2011, Page 107-116 PDF (186.73 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/besps.2011.35974 | ||||
View on SCiNiTO | ||||
Authors | ||||
Yousry Hussien* 1; Amal Gharib1; Hend Ibrahim1; Mohamed Abdel-Ghany2; Wael Elsawy3 | ||||
1Department of Medical Biochemistry, Faculty of Medicine, Zagazig University, Zagazig, Egypt. | ||||
2Department of General Surgery, Faculty of Medicine, Al-Azhar University, Cairo, Egypt | ||||
3Department of Oncology, Faculty of Medicine, Zagazig University, Zagazig, Egypt | ||||
Abstract | ||||
Mutations in breast cancer susceptibility genes 1 and 2 (BRCA1 and BRCA2) have higher frequency in breast cancer cases. Three founder mutations 185delAG and 5382insC in BRCA1 and 6174delT in BRCA2 are frequently reported in breast cancer patients from various ethnic backgrounds. The aim of the current study was to evaluate the frequency of the BRCA1 and BRCA2 mutations in Eastern Egyptian sporadic breast cancer patients and their relatives as possible diagnostic markers. One hundred women with sporadic breast cancer and one hundred healthy firstdegree relatives were included in the study. Multiplex polymerase chain reaction method was used to analyze the DNA prepared from peripheral blood. Data analysis showed that 185delAG mutation in BRCA1 was expressed at low frequency (3%), whereas the 5382insC in BRCA1 and 6174delT in BRCA2 were not detected within the Eastern Egyptian population. Conclusion: The low percentage of BRCA1 and BRCA2 mutations in apparently sporadic early- onset cancer and relatives suggested that mutation detection is insufficient to screen Egyptian population. | ||||
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