A study on the effect of Chromium Administration on the Altered Urinary Bladder Reactivity in Experimentally-induced Diabetic Rats | ||||
| Bulletin of Egyptian Society for Physiological Sciences | ||||
| Article 17, Volume 31, Issue 1, December 2011, Page 239-260 PDF (1.01 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/besps.2011.35985 | ||||
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| Author | ||||
| M. Bendary* | ||||
| Physiology Department, Faculty of Medicine, Minoufiya University Egypt | ||||
| Abstract | ||||
| Diabetic-induced urinary bladder dysfunction (diabetic cystopathy) is among the most common complications of chronic uncontrolled diabetes mellitus. This cystopathy, involves altered urinary bladder contractility and reactivity, is suggested to be due to the oxidative stress encountered in diabetes. Chromium, an antioxidant micronutrient, is proposed nowadays as an adjuvant in some diabetic complications. Accordingly, this study was designed to evaluate the possible role of oral chromium administration on the altered urinary bladder reactivity in experimentally-induced diabetic rats. In the present study, 36 adult male albino rats, weighing 150-180 gm each, were used. They were divided into 6 equal groups. Group1 (Control group), Group2 (Cr-treated control group), Group3 (untreated diabetic group), Group4 (Insulin-treated diabetic group), Group5 (Cr-treated diabetic group) and Group6 (Concomitantly insulin & Cr-treated diabetic group). Fasting serum glucose, serum insulin, homeostatic model assessment to detect insulin resistance (HOMA-IR index), lipid profile, urinary bladder tissue malondialdehyde (MDA) (a tissue marker of oxidative stress and lipid peroxidation) and glutathione (GSH) (as an index of the tissue antioxidant enzyme defense activity) were measured. In addition, the total body weight, urinary bladder weight and the reactivity of isolated urinary bladder strips to carbachol (Cch) were determined. The untreated diabetic group exhibited significant increase of the serum glucose, HOMA-IR index, lipid profile, bladder tissue MDA and urinary bladder weight, (p<0.05) together with a significant decrease (p<0.05)of serum insulin, total body weight and bladder tissue GSH level. In addition, the contractile response of the isolated urinary bladder strips to Cch was significantly higher in untreated diabetic rats relative to the other tested groups, (p<0.05). Interestingly, when the diabetic rats were treated with subtherapeutic doses of insulin alone or oral chromium alone, a significant improvement (p<0.05) of all the altered parameters was obtained. However, when the diabetic rats were administered concomitantly with insulin & chromium, a highly significant improvement (p<0.001) of the deteriorated parameters that have been returned nearly close to the control level. In conclusion, oral chromium administration has a beneficial effect in ameliorating the changes in the urinary bladder reactivity in experimentally-induced diabetic rats, probably through its antioxidant effect and its ability in increasing insulin sensitivity. | ||||
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