Molecular Detection of Mutated Factor V (Leiden) and Factor XIII in Ischemic Heart Disease | ||||
| Bulletin of Egyptian Society for Physiological Sciences | ||||
| Article 18, Volume 31, Issue 1, December 2011, Page 261-270 PDF (195.74 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/besps.2011.35987 | ||||
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| Authors | ||||
| Olfat Shaker* 1; Ahmed El-Demery2; Mohamed El Nemr3; Ahmed Desoky4 | ||||
| 1Biochemistry Department, Faculty of Medicine, Cairo University | ||||
| 2Biochemistry Department, Faculty of Medicine, October 6 University | ||||
| 3Internal Medicine Department, Faculty of Medicine, October 6 University | ||||
| 4Physiology Department, Faculty of Medicine, October 6 University | ||||
| Abstract | ||||
| The present work was performed to evaluate the incidence of gene mutation affecting factor V Leiden (FVL) &factor XIII in cases of myocardial ischemia. Thirty cases of myocardial ischemia as well as ten age and sex matched healthy subjects were included in the study. DNA was extracted from blood samples drawn from all subjects included in the study and was subjected to PCR amplification for factors V (Leiden) and factor XIII genes, using biotinylated specific primers. The PCR products were hybridized using strip containing immobilized allele-specific oligonucliotide probe. The products of hybridization were visualized using striptavidin-alkaline pH and color substrate. LDL-c was significantly higher in heterozygous factor V leiden The results of the study showed that heterozygous mutations involving factor V Leiden cases were in a positive correlation with LDL-c results and homozygous mutations involving factor XIII cases showed a positive correlation with AST changes. These results gave an idea about the mutational changes involving factors V Leiden and XIII and may be considered as risk factors for the incidence of myocardial infarction. | ||||
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