Comparative Study on the ameliorative effect of Indian Costus Extract versus Omeprazole on Fundic Mucosa in Ulcer model.

Mohamed, Sara Adel Hosny; Yacoub, Mira Farouk Youssef; Fasseh, Alaa Essam-Eldin Ali; Fares, Amal Elham Ahmed

doi:10.21608/ejh.2024.275752.2046

	Comparative Study on the ameliorative effect of Indian Costus Extract versus Omeprazole on Fundic Mucosa in Ulcer model.
Egyptian Journal of Histology
Articles in Press, Accepted Manuscript, Available Online from 11 June 2024
Document Type: Original Article
DOI: 10.21608/ejh.2024.275752.2046
View on SCiNiTO
Authors
Sara Adel Hosny Mohamed ¹; Mira Farouk Youssef Yacoub ²; Alaa Essam-Eldin Ali Fasseh ³; Amal Elham Ahmed Fares ⁴
¹Histology department, faculty of medicine, cairo university
²Histology Department, Faculty of Medicine, Cairo University.
³Histology Department, Faculty of Medicine, Cairo University, Egypt
⁴Histology department, Kasr Alaini, Faculty of Medicine, Cairo University
Abstract
Background: Gastric ulceration disease is of major global health burden which lead to serous complications if left without treatment. This study aimed at evaluating the ameliorative effect of Indian costus ethanolic extract versus omeprazole as the most recently used ulcer therapy in aspirin induced peptic ulcer. Methods: twenty nine rats divided into: Group І (Control group) which is sub divided into subgroups (distilled water, omeprazole control recieved omeprazole for 10 days, saussurea control recieved saussurea for 10 days), Group II: subgroup IIa (Aspirin; ASA diseased), subgroup IIb (Recovery), Group III: subgroup IIIa (omeprazole; OMZ), subgroup IIIb (saussurea costus; S costus). Asprin given daily orally for 5 days to all groups. After 5 days, group IIIa received Omeprazole, group IIIb received S costus for 10 days. Serum MDA TNF-α, AST and frozen gastric NO, PGE2 were measured. Fundic sections were subjected to histological. immunohistochemical and morphometric analysis. Results: Groups (IIa& IIb) revealed marked loss of gastric lining with cells having dark pyknotic nuclei. A significant increase in mean values of MDA, TNF-α, AST, mean area % of COX2 & NFκB immunopositivity with decrease in mean area % of PAS in group IIa&IIb compared to other groups. Omeprazole and S costus group ameloirated the biochemical, histological and immunohistochemical changes induced by aspirin on fundic mucosa. Conclusion: Aspirin can lead to metaplastic changes on the cellular level in the fundic mucosa. S costus produced comparable results to omeprazole with significant improvement in fundic mucosa in gastric ulcer rat model and the liver functions showed improvment.
Keywords
Keywords: Omeprazole; saussurea costus extract; Aspirin intestinal metaplasia; COX-2; NFκB


Statistics Article View: 23