Effect of Vitamin D on the Spleen of Adult Male Rats Fed on Diet with High Fat: A Histological and Immunohistochemical Study | ||||
| Egyptian Journal of Histology | ||||
| Article 16, Volume 42, Issue 4, December 2019, Page 1001-1017 PDF (15.47 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2019.13030.1120 | ||||
 View on SCiNiTO | ||||
| Author | ||||
Heba Kamal Mohamed 
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| Department of Human Anatomy and emberyology- Faculty of Medicine- Assiut University- Egypt | ||||
| Abstract | ||||
| Background: High fat diet (HFD) is the cause of obesity prevalence which is a serious health problem. Vitamin D is considered a very important modulator in cellular inflammation and immune systems. Aim of the work: To demonstrate the histological changes in the spleen of the adult male rats fed with HFD and to evaluate vitamin D role in counteracting the harmful effects of HFD on the spleen. Materials and Methods: A total number of 36 male adult albino rats which were classified equally and randomly into three groups as follows; Control group where rats were administered the control diet for a period of 10 weeks, HFD fed group which was administered HFD for 10 weeks and HFD + vitamin D group which was administered HFD concomitantly with vitamin D orally daily for 10 weeks at a dose of 1 ug (40 IU)/kg. Samples from the spleen were prepared for histological and immunohistochemical study. Results: The group received HFD revealed marked histological alterations with lymphatic follicles proliferation. Most splenic cells showed degenerative changes. Deposition of hemosidrin pigments, markedly dilated blood sinusoids and blood extravasation were observed. CD86 had a strong cytoplasmic expression in dendritic cells. Moreover, cytoplasmic immune reaction for iNOS and caspase-3 in the white and red pulps of the spleen was intense. Vitamin D co-adminstration greatly preserved the histological and immunohistochemical structure of the spleen. Conclusion: This study suggests that vitamin D has a role in splenic protection and can attenuate the deleterious effects commonly detected in the spleen and immune system in case of obesity induced by HFD. | ||||
| Keywords | ||||
| CD86; Obesity; spleen; Vitamin D | ||||
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