Study of the Role of Migration Inducting Gene 7 as a Molecular Marker for Early Detection of Endometrial Carcinoma Micrometastasis.

Mahmoud, Dina Aly Abdelaziz; Seleem, Amal Kamel; El Refaeey, Abdel Aziz Abdel aleem; Barakat, Lamiaa abdelatef; Tawfik, Mohamed Mahmoud

doi:10.21608/ajbas.2024.292248.1213

	Study of the Role of Migration Inducting Gene 7 as a Molecular Marker for Early Detection of Endometrial Carcinoma Micrometastasis.
Alfarama Journal of Basic & Applied Sciences
Volume 5, Issue 3, July 2024, Page 381-394 PDF (789.22 K)
Document Type: Original Article
DOI: 10.21608/ajbas.2024.292248.1213
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Authors
Dina Aly Abdelaziz Mahmoud ¹; Amal Kamel Seleem²; Abdel Aziz Abdel aleem El Refaeey³; Lamiaa abdelatef Barakat⁴; Mohamed Mahmoud Tawfik⁵
¹department of chemistry, faculity of science, portsaid university
²Biochemistry, Faculty of medicine, Mansoura University.
³Obstetrics and Gynecology, Faculty of Medicine, Mansoura University.
⁴Biochemistry, Faculty of Science, Port-Said University.
⁵biotechnology, Faculty of Science, Port-Said University.
Abstract
Background and objectives: Endometrial carcinoma diagnosed early by abnormal uterine bleeding especially in postmenopausal women. Migration-Inducting Gene 7 (MIG-7) is a cysteine-rich protein first identified in endometrial cancer cells upon hepatocyte growth factor (HGF) treatment. Subjects and methods: This case-control study was carried out in Mansoura University, Egypt on a total of 75 patients with 25 age-matched healthy female volunteers as control group and 50 patients classified according to standard criteria based on data of International Federation of Gynecology and Obstetrics (FIGO) into two groups (non-metastatic endometrial carcinoma and metastatic endometrial carcinoma). Total RNA extracted from peripheral blood, cDNA synthesized from RNA and Real-time PCR done for quantification of MIG7. Results: Comparison between the control group, patient with non-metastatic endometrial carcinoma and patient with metastatic endometrial carcinoma with regard to MIG7 gene expression reveal highly significant differences ((0.69±0.21), (1.38±0.39) and (1.97±0.45)) respectively, P-values (all˂ 0.001). AUC values for MIG7 equal 0.945 while MIG7 show sensitivity percentage of 88% and 88% specificity percent.In the univariable analysis MIG7 gives an odds ratio (ORs) 1.077 at p-values of 0.002 while in the multivariable analysis demonstrate elevated ORs, p-values (0.024).
Keywords
endometrial carcinoma; gene expression; MIG7


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