RANTES, TNF-α, Oxidative Stress and Hematological Abnormalities in Hepatitis C Virus Infection. | ||||
| Bulletin of Egyptian Society for Physiological Sciences | ||||
| Article 13, Volume 31, Issue 2, June 2011, Page 167-178 PDF (268.91 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/besps.2011.36135 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Gamil Tawadrous* 1; Amal Aziz2; Dalia Amin2; Ahmed Eldemery3; Mostafa Mostafa4 | ||||
| 1Department of Medical Biochemistry, Faculty of Medicine, Cairo University | ||||
| 2Department of Clinical & Chemical Pathology, Faculty of Medicine, Cairo University | ||||
| 3Department of Medical Biochemistry, Faculty of Medicine, October 6 University | ||||
| 4Department of Internal Medicine, Faculty of Medicine, October 6 University | ||||
| Abstract | ||||
| Chronic infection with hepatitis C virus (HCV) is associated with failures of T-cell– mediated immune clearance and with abnormal B-cell growth and activation.HCV infection is characterized by a systemic oxidative stress that is most likely caused by a combination of chronic inflammation, iron overload, liver damage, and proteins encoded by HCV. Following viral infection, multiple pro-inflammatory mediators contribute to recruitment of immune cells to the liver and to the generation of an antiviral immune response. Recent publications mark chemokines and their receptors as key players in leukocyte recirculation through the inflamed liver. The present study involved 75 male subjects, included into two groups: Group1 (n=30) control group; group II (n=45) patients with chronic HCV. For all subjects the following investigations were performed: estimation of the levels of bilirubin, albumin, prothrombin concentration, glycosylated hemoglobin (HbA1C), creatinine, α- fetoprotein (AFP), HCV RNA, and activities of alanine and aspartate transaminases (ALT& AST) as well as alkaline phosphatase. Also, Regulated on Activation Normal T Cell Expressed and Secreted (RANTES), tumor necrosis factor alpha (TNF-α), malondialdehyde (MDA) and nitric oxide (NO) were assessed. Plasma HCV-RNA concentration (viral load) was determined by real time PCR step one using Applied Biosystem. Complete blood picture was assayed using Abbott cell dyn 3700 hematology analyzer. There were significant increase of the levels of RANTES,TNF- α, MDA and NO in HCV infected patients compared with control group (P <0.05) and in these patients, these levels showed significant positive correlation with the HCV RNA viral load. Also, mild leucopenia, thrombocytopenia, neutropenia, lymphocytosis, with consequent significant increase in the lymphocytes / neutrophils (L/N) ratio were detected in these patients. Conclusion: The data support the concept of chemokines (RANTES) as mediators of liver cell injury in hepatitis C infection. In addition, MDA and NO levels might be used as monitoring markers for oxidative stress in hepatitis C infection. | ||||
| Keywords | ||||
| Chronic liver disease; RANTES, TNF-α, nitric oxide, MDA, Real time PCR, and complete blood picture | ||||
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