Methylenetetrahydrofolate Reductase C677T Polymorphism and Plasma Homocysteine Levels in Egyptian Patients with Primary Open-angle Glaucoma | ||||
Bulletin of Egyptian Society for Physiological Sciences | ||||
Article 20, Volume 31, Issue 2, June 2011, Page 263-275 PDF (268.09 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/besps.2011.36146 | ||||
View on SCiNiTO | ||||
Authors | ||||
Wafaa Emam* 1; Bahaa Hassan2 | ||||
1Medical Biochemistry Department , Faculty of Medicine Zagazig University | ||||
2Ophthalmology Department, Faculty of Medicine Zagazig University | ||||
Abstract | ||||
Objectives: The association between primary open-angle glaucoma (POAG) and cardiovascular disease has recently stimulated interest in the role of homocysteine in the pathogenesis of glaucoma. In the present study, we aimed to measure plasma homocysteine and its relation to C677T methylenetetrahydrofolate reductase polymorphism in patients with POAG. Subjects and Methods: Fasting venous blood samples from one hundred patients with POAG and 50 age and sex matched healthy control subjects were analyzed for plasma homocysteine by high performance liquid chromatography (HPLC) and genotype for C677T polymorphism by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: There was no statistically significant difference in POAG patients and controls for the age and sex (P>0.05). Mean plasma homocysteine was significantly higher in POAG patients (12.37±3.05μmol/l.) compared with controls (10.40 ±1.74 μmol/l.) (P< 0.001). C677T polymorphism did not show significant differences between the POAG patients and control subjects (CC: 46%, CT: 41%, TT: 13%) for patients with POAG; CC: 60%, CT: 32:%, TT: 8% for controls) (P>0.05). Frequency of T allele was 33.5% and 24% for patients with POAG and controls, respectively. Conclusion: In Egyptian patients plasma homocysteine was elevated in POAG patients but no association was found between C677T polymorphism and POAG patients. If homocysteine measurement proves useful for risk stratification, therapies that lower it may aid the management of POAG. | ||||
Keywords | ||||
Primary open-angle glaucoma; Homocysteine; gene C677T polymorphism | ||||
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