Biochemical Studies on the Molecular Level of Polioviruses | ||||
| Bulletin of Egyptian Society for Physiological Sciences | ||||
| Article 8, Volume 30, Issue 1, December 2010, Page 137-148 PDF (237.29 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/besps.2010.36169 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Hend El Sherbini* 1; Naglaa Abd El Meguid2 | ||||
| 1Clinical Pathology Department, Faculty of Medicine, Cairo University | ||||
| 2The Holding Company for Biological Products and Vaccines (VACSERA) | ||||
| Abstract | ||||
| Paralytic poliomyelitis continues to be an important disease affecting many children. The strategy for poliomyelitis eradication includes routine immunization, surveillance of acute flaccid paralysis (AFP) and mopping-up activities. Environmental monitoring, mainly involving the screening of waste water can provide evidence of the circulation or absence of wild-type polioviruses. The present work has been carried out to investigate the sequence divergence of neurovirulent derivatives of the Sabin oral poliovirus vaccine (OPV) strains, to establish epidemiological link between isolates, and to determine poliovirus genotype which circulates in Egypt. Enzyme Linked Immunoassay (ELIZA) and Reverse Transcription Polymerase Chain Reaction (RT-PCR) for intratypic differentiation have been used. Molecular analysis of the VPI/2A junction region has been performed on 22 isolates using the dideoxy chain termination method. Sequencing of the amplified region has been performed using the automated genetic analyzer ABI prism 310. Forward and reverse electropherograms were compared using sequencing alignment software version 2. Phylogenetic analysis of the VPI/2A region was done using Clustal X software. | ||||
| Keywords | ||||
| Sequence divergence; neurovirulent; epidemiological link; genotype; VPl/2A junction region; Phylogenetic analysis | ||||
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