Schistosoma Mansoni: Partial Molecular Characterization of the Gene Encoding Zinc Finger Protein of the Lung Stage (7-Days Schistosomula) | ||||
| Bulletin of Egyptian Society for Physiological Sciences | ||||
| Article 11, Volume 30, Issue 1, December 2010, Page 171-184 PDF (279.81 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/besps.2010.36172 | ||||
 View on SCiNiTO | ||||
| Author | ||||
| Samir Mahgoub* | ||||
| Department of Biochemistry, Faculty of Medicine, Al-Minia University | ||||
| Abstract | ||||
| Schistosomiasis is a serious parasitic disease with world-wide distribution, causing an estimated 200 000 deaths per year. Despite the fact that the global distribution of schistosomiasis has changed significantly in the past 50 years, particularly in regions where control strategies have been successfully employed, the disease remains endemic in over 70 developing countries and more than 200 million people are estimated to be harboring the disease. Chemotherapy, although effective, it does not prevent re-infection, and in addition, partial drug resistance may occur. Hence, immunological intervention in the form of a vaccine would contribute to the success of the present efforts. Most of the trials in the development of anti-schistosomiasis vaccine were involving membrane-associated antigens contained in the adult Schistosoma mansoni (S. mansoni) tegument because they are capable of stimulating protective immunity. In the current study, a trial to obtain antigen varieties which could be vaccine candidates by incorporating internal antigens of the lung stage of S. mansoni (7-days schistosomula) in addition to the tegumental antigens was done. The soluble extract of the lung stage was obtained by sonicating the whole parasite, then, coupled to Sepharose-4B column for affinity purification of pooled sera collected from chronically infected patients. The purified sera were used to immunoscreen λgt 11 cDNA library of 7-days schistosomula. The plaques purification after three rounds of immunoscreening gave a number of cDNA clones. one of the isolated clones (clone 2-4) was amplified by PCR using λgt 11 forward and reverse primers, then ,cloned in a plasmid vector (PCRTMII). The cloned insert was partially sequenced 270 bp from the 5/- end using Sp6 primer as well as 187 bp from the 3/-end using T7 primer. The sequenced part of the clone showed it has two open reading frames (ORFs) with 31-36% homology to the gene that encodes Zinc Finger protein (the transcriptional regulatory protein) from a number of eukaryotic species including human , rat and mice. | ||||
|
Statistics Article View: 102 PDF Download: 177 |
||||
