Ameliorative effect of resveratrol against type 2 diabetes in rats through triggering of autophagy and inhibition of mTOR pathway. | ||||
| Bulletin of Egyptian Society for Physiological Sciences | ||||
| Volume 44, Issue 3, July 2024, Page 150-168 PDF (350.64 K) | ||||
| Document Type: Review Article | ||||
| DOI: 10.21608/besps.2024.279336.1166 | ||||
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| Authors | ||||
Lina Abdelhady Mohammed 1; Ahmed Medhat Hegazy  2; Walaa Bayoumie El Gazzar 1; Noha Osama El-Shaer 3; Heba Bayoumi 4; Salwa A. Elgendy 5; Heba A. Elnoury 5; Hend Elsayed Nasr 1
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| 1Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Benha University, Benha 13518, Egypt. | ||||
| 2Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh 13736, Qalyubia, Egypt. | ||||
| 3Department of Physiology, Faculty of Medicine, Benha University, Benha 13518, Egypt. | ||||
| 4Department of Histology and Cell Biology, Faculty of Medicine, Benha University, Benha 13518, Egypt. | ||||
| 5Deparment of Pharmacology, Faculty of Medicine, Benha University, Benha 13518, Egypt. | ||||
| Abstract | ||||
| Background: Type 2 diabetes mellitus (T2DM), which affects millions of individuals, has become a serious health problem. The mammalian target of rapamycin (mTOR) hyperactivation is a negatively involved autophagy that may in fact aid in the loss of β-cell function. Resveratrol (RSV) is a naturally occurring phytoalexin, mostly found in cereals, fruits, and vegetables that have antioxidant effects. Aim: In the current work, Wistar rats exposed to high-fat diet (HFD) and streptozotocin (STZ)-induced T2DM were evaluated for resveratrol's protective effects on pancreatic functions, and mTOR-mediated autophagy. Materials and Methods: Four equal groups of forty male Wistar rats were created at random. Group 1 (G1) was maintained in typical control circumstances and given a balanced diet; G2, G3, and G4 were fed HFD for 10 weeks continuously; at the end of the 6th week injected I/P STZ as a single dose to induce T2DM. Three days after diabetic induction, G3 was received RSV daily for continuously 4 weeks. G4 was received RSV in addition to Chloroquine (CQ) as autophagy inhibitor. Results: The findings shown that RSV reduces the pancreatic dysfunctions brought on by HFD-STZ by inhibiting the mTOR pathway through decreasing the level of mTOR, Cas-3, and p62 and increasing the level of LC3. RSV activate autophagy and inhibit apoptotic-mediated cell death confirmed by transmission electron micrograph findings. Conclusion: RSV is thought to protect pancreatic cells by inhibiting the mTOR pathway, which regulates the transition between autophagy and apoptotic machinery in diabetic circumstances. | ||||
| Keywords | ||||
| autophagy; mTOR pathway; oxidative stress; resveratrol; type 2 diabetes mellitus | ||||
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