Thrombophilic Genes Mutations in Preeclampsia | ||||
Bulletin of Egyptian Society for Physiological Sciences | ||||
Article 2, Volume 30, Issue 2, June 2010, Page 21-34 PDF (493.65 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/besps.2010.36295 | ||||
View on SCiNiTO | ||||
Authors | ||||
Gamal Othman* 1; Abd Elnaser badawy1; Rizk Elbaz2; Ahmad Ragab3 | ||||
1Medical Biochemistry Dept. , Mansoura Faculty of Medicine | ||||
2Genetic Unit of Pediatric Dept. , Mansoura Faculty of Medicine | ||||
3Gynecology and Obstetric Dept., Mansoura Faculty of Medicine | ||||
Abstract | ||||
Background: Preeclampsia is a multisystem disorder involving vasoconstriction and hypertension in the mother and decreased blood flow. There are inconsistent reports on whether there is an association between preeclampsia and thrombophilia. The Aim of the Study: The present study aimed to determine the relationship of mutations of factor V Leiden, prothrombin and methylene tetrahydrofolate reductase (MTHFR) genes in Egyptian preeclamptic patients. Materials and Methods: Fifty six preeclamptic women and 48 normal pregnant women were tested for detection of mutations of factor V Leiden (FVL), prothrombin and methylene tetrahydrofolate reductase genes by genotyping using multiplex allele specific PCR, restriction enzymes and agarose gel electrophoresis. Results: There was significant association between mutations of factor V Leiden gene in preeclamptic patients (8 positive cases out of 56) compared with control cases (No positive cases out of 48), prothrombin gene in preeclamptic patients (22 positive cases out of 56) compared with control cases (only one positive case out of 48), while, no significant association between mutations of methylene tetrahydrofolate reductase gene C677T or A1298C in preeclamptic patients when compared with control cases. Conclusion: This study suggests the existence of a linkage between FVL, prothrombin genes polymorphism but not MTHF reductase genes polymorphism in the pathogenesis of preeclampsia. | ||||
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