Dysregulation of Some Skeletal Muscle miRNAs in High-Fat Diet-Induced Obesity: Implications for Metabolic Disorders.
Youssef, E., Elfiky, A., Ramadan, L. (2024). Dysregulation of Some Skeletal Muscle miRNAs in High-Fat Diet-Induced Obesity: Implications for Metabolic Disorders.. EKB Journal Management System, 67(12), 443-450. doi: 10.21608/ejchem.2024.292372.9765
Elham M. Youssef; Asmaa M. Elfiky; Lamiaa M. Ramadan. "Dysregulation of Some Skeletal Muscle miRNAs in High-Fat Diet-Induced Obesity: Implications for Metabolic Disorders.". EKB Journal Management System, 67, 12, 2024, 443-450. doi: 10.21608/ejchem.2024.292372.9765
Youssef, E., Elfiky, A., Ramadan, L. (2024). 'Dysregulation of Some Skeletal Muscle miRNAs in High-Fat Diet-Induced Obesity: Implications for Metabolic Disorders.', EKB Journal Management System, 67(12), pp. 443-450. doi: 10.21608/ejchem.2024.292372.9765
Youssef, E., Elfiky, A., Ramadan, L. Dysregulation of Some Skeletal Muscle miRNAs in High-Fat Diet-Induced Obesity: Implications for Metabolic Disorders.. EKB Journal Management System, 2024; 67(12): 443-450. doi: 10.21608/ejchem.2024.292372.9765

Dysregulation of Some Skeletal Muscle miRNAs in High-Fat Diet-Induced Obesity: Implications for Metabolic Disorders.

Egyptian Journal of Chemistry
Volume 67, Issue 12, December 2024, Page 443-450  XML PDF (562.09 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2024.292372.9765
View on SCiNiTO View on SCiNiTO
Authors
Elham M. Yousseforcid 1; Asmaa M. Elfiky2; Lamiaa M. Ramadan email orcid 1
1Biochemistry Department, National Research Centre, Cairo, Egypt, 12622
2Environmental and Occupational Medicine Department, Environmental and Climate Changes Research Institute, National Research Centre, Cairo, Egypt, 12622
Abstract
Abstract

Skeletal muscle-adipose tissue crosstalk is crucial for developing therapeutic strategies for various metabolic disorders. While obesity is known to disrupt microRNA (miRNA) expression profiles in skeletal muscle, a comprehensive understanding of this phenomenon remains elusive. Therefore, our study aims to investigate the impact of high-fat diet (HFD)-induced obesity on miRNA dysregulation within skeletal muscle tissue at distinct time points. In HFD model, rats received HFD for 2, 4, 6, 8, or 10 weeks. Body weight was determined at 2, 4, 6, 8, and 10 weeks. At the end of each interval, group of animals (n = 8) were sacrificed and skeletal muscle tissues were harvested to assess miRNAs expression levels. HFD administration for 8 and 10 weeks resulted in marked weight changes in comparison to control group. There were not much significant changes in body weight seen in low durations of HFD feeding. Alterations in miR130a, miR30a-5p, miR133a-5p, miR193a-5p, and miR125a-5p expression levels were observed at different time point relative to control rats. While miR let-7 and miR107-5p were upregulated at all-time points compared to control animals. Thus, skeletal muscle miRNA dysregulation likely plays a role in HFD-induced obesity.
Keywords
High-fat diet; Obesity; microRNAs; Skeletal muscles; Expression profiling; Body weight
Statistics
Article View: 303
PDF Download: 206