The Role of Some Antioxidants in Diabetes Mellitus Induced in Rats | ||||
| Bulletin of Egyptian Society for Physiological Sciences | ||||
| Article 4, Volume 30, Issue 2, June 2010, Page 47-64 PDF (313.53 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/besps.2010.36298 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Nagy Tawfek* 1; Mahmoud Elrehany2; Hanaa Hassan1 | ||||
| 1Department of Zoology, Faculty of Science , El-Minia University, El-Minia, Egypt | ||||
| 2Department of Biochemistry, Faculty of Medicine, El-Minia University, El-Minia, Egypt | ||||
| Abstract | ||||
| The aim of the present study was to examine the involvement of oxidative stress in the progression of pancreatic β-cell dysfunction in type 1 diabetes and to evaluate the potential usefulness of some antioxidants supplementation in the treatment of type 1 diabetes. The severity of diabetes in the different groups has been studied in relation to the level of cytokines released during the oxidative stress. The present study was achieved using 24 male Sprague Dawley albino rats. Rats were divided into three groups: normal control rats, diabetic control rats, and diabetic rats received mixture of antioxidants. A mixture of antioxidants [N-acetyl-cysteine (NAC), alpha-lipoic acid (LA), vitamin E and vitamin C] was orally administered daily to cyclophosphamideinduced diabetic rats for a period of two months. The results revealed that diabetic rats had significant increase in tumor necrosis factor-α (TNF-α) concentration and transcription nuclear factor-kappa beta (NF-кβ) concentration, as compared to normal control rats. After treatment of diabetic rats with the antioxidants for two months, tumor necrosis factor-α (TNF-α) and nuclear factor-kappa beta (NF-кβ) concentrations showed a highly significant decrease (p< 0.001) when compared with the diabetic control group. Histological analysis of the pancreas revealed that the antioxidants treatment preserved the normal morphology of Islets of pancreas, and β- cell mass when compared with diabetic rats. The combination of these antioxidants was more effective in suppression of apoptosis which was associated with the development of type 1 diabetes. Immunohistochemical analysis indicated that antioxidants protect β-cell from cytokine induced dysfunction and death through inhibition of specific nuclear factor –кβ activity which was more visible in the nuclei of Islet cells in diabetic rats than antioxidants-treated rats. On the basis of the present results it could be concluded that [N-acetyl-cysteine (NAC), alpha-lipoic acid (LA), vitamin E and vitamin C] restored the activities of the above parameters in different ways, depending on special mechanism in each one. Supplementation of antioxidants at once after diagnosis of diabetes may delay the complications of diabetes. This finding suggests a potential usefulness of antioxidants for treating diabetes and provides further support for the implication of oxidative stress in β-cell dysfunction in diabetes by providing protection against hyperglycemia. | ||||
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