Antagonistic Effects of Selenium (Se) and Vitamin C against the Hepatotoxic Effects of AFB l in Rats | ||||
| Bulletin of Egyptian Society for Physiological Sciences | ||||
| Article 6, Volume 30, Issue 2, June 2010, Page 87-110 PDF (389.14 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/besps.2010.36301 | ||||
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| Authors | ||||
| Salwa Abaskhroun* 1; Hala Hamouda1; Ayman Wagih1; Rania Emam1; Mona Abd El-Azem2 | ||||
| 1Medical Biochemistry Department, Faculty of Medicine, Tanta University | ||||
| 2Pathology Department, Faculty of Medicine, Tanta University | ||||
| Abstract | ||||
| Objective: Aflatoxins (AFs) a group of mycotoxins, are produced by the filamentous fungi Aspergillus, particularly flavus and parasiticus. Aflatoxin B1 (AFB1) is the most prevalent and the most potent of these toxins, which has potent hepatotoxic and hepatocarcinogenic properties in animals and humans. Because of the wide spread of AFB1 contaminated food and feeds and because of its hepatotoxicity, the present experimental study was carried out. Aim of the study: The aim of the present study was to highlight the antagonistic effects of selenium (Se) and vitamin C against the hepatotoxic effect of AFB1 as they have a role in prevention of formation of carcinogens from precursor compounds and they are natural antioxidants.Materials and methods: The study was carried out on 85 white male albino rats divided into; Group I (control group):10 rats received I.P injection of dimethylsulfoxide (DMSO) for 15 days. Group II: 45 rats received I.P injection of AFB1 for 15 days and then subdivided into three equal subgroups; Group II a: received regular diet, group II b: received Se orally for 15 days. Group II c: received vitamin C orally for 15 days. Group III: received Se orally for 15 days during and 15 days after AFB1 injection. Group IV: received vitamin C orally for 15 days during and 15 days after AFB1 injection. All groups were subjected to measurements of the following; liver function tests, serum & liver tissue levels of malondialdehyde (MDA), reduced glutathione (GSH), and the activity of serum &liver tissue glutathione S- transferase enzyme (GST) and paraoxonase1 (PON1) enzymes. Liver specimens were examined histopathologically. Results: The present study confirmed the hepatotoxicity of AFB1, as marked by the significant increase of serum AST, ALT enzymes activities and decrease serum albumin which is confirmed by histopathological study of liver tissues. Serum and liver tissue MDA levels were significantly increased in AFB1 treated animals. There was significant increase of the inducible enzyme GST activity and significant decrease of GSH level in AFB1 treated groups. There was significant decrease in PON1 enzyme activity in both serum and hepatic tissue. Se and vitamin C were effective only when given with and after the xenobiotic treatment for another 15 days. They caused significant decrease of serum activity of AST, ALT and increase in serum albumin. They also caused a significant decrease in MDA level and GST enzyme activity with significant increase of GSH level and PON1 enzyme activity in both serum and liver tissues. Conclusion: All the above findings confirm the protective role of Se and vitamin C on the hepatotoxicity caused by AFB1. | ||||
| Keywords | ||||
| Selenium (Se); Malondialdehyde (MDA); reduced glutathione (GSH); glutathione S- transferase enzyme (GST) paraoxonase1 (PON1) | ||||
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