Comparative Study of the Protective Effects of Taurine and Melatonin on Cytochrome P450 2E1 and some Oxidative Stress Markers in Streptozotocin-induced Diabetic Rats | ||||
| Bulletin of Egyptian Society for Physiological Sciences | ||||
| Article 10, Volume 30, Issue 2, June 2010, Page 165-182 PDF (486.84 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/besps.2010.36306 | ||||
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| Authors | ||||
| Manal El-Batch* 1; Azza Hassan2; Heba Mahmoud3 | ||||
| 1Medical Biochemistry Department, Faculty of Medicine, Tanta University | ||||
| 2Microbiology & Immunlogy Department, Faculty of Medicine, Tanta University | ||||
| 3Pharmacology Department, Faculty of Medicine, Tanta University | ||||
| Abstract | ||||
| Melatonin and taurine have alleviative effects in streptozotocin (STZ)-induced diabetic rats. Male Wistar rats were divided into non-diabetic, diabetic, diabetic melatonin (administered at 200 μg/kg/day dissolved in 0.5 ml of normal saline) and diabetic taurine (administered at 2% in the drinking water) supplemented groups. At the end of the study, blood was collected and used for determination of glucose, total cholesterol, triglyceride levels, liver enzymes (ALT and AST), β-hydroxybutyrate (β- HB), in addition to advanced oxidation protein product (AOPP) level, and paraoxonase (PON1) enzyme activity. Also, liver tissue was examined for malondialdhyde (MDA) level, glutathione peroxidase (GPx) enzyme activity and cytochrome P450 2E1 (CYP2E1) both enzyme activity and gene expression. Light microscopy pictures of liver tissue were also evaluated for signs of its damage. An increased CYP2E1 activity and gene expression with a concomitant significant change in oxidative stress parameters were found in STZ-induced diabetic rats, suggesting the possible diabetes-induced injury. Taurine or melatonin supplementation to the diabetic rats alleviated these experimental parameters with more significant effect for taurine than that of melatonin. Suppression of β-HB production by taurine can be one of the mechanisms of reduction in CYP2E1. Conclusion: Taurine has the capabilities more than melatonin in protecting the liver from the hepatic injury induced by type 1 diabetes, by reducing the oxidative stress and restoring CYP2E1 activity and gene expression, suggesting hepatic protective nature of taurine in diabetic rats. Therefore antioxidants might prove beneficial as an adjuvant treatment to insulin in type 1 diabetes associated with manifestations of liver injury. | ||||
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