Circulating of is/1494-like strains of IBV in vaccinated commercial broiler flocks in Egypt during years 2012 to 2014
SA, A., HA, H., A, A., Zanaty, A., Shalaby, M. (2016). Circulating of is/1494-like strains of IBV in vaccinated commercial broiler flocks in Egypt during years 2012 to 2014. EKB Journal Management System, 62(2), 29-35. doi: 10.21608/vmjg.2016.363155
Abdelghany SA; Hussein HA; Arafa A; Ali Zanaty; MA Shalaby. "Circulating of is/1494-like strains of IBV in vaccinated commercial broiler flocks in Egypt during years 2012 to 2014". EKB Journal Management System, 62, 2, 2016, 29-35. doi: 10.21608/vmjg.2016.363155
SA, A., HA, H., A, A., Zanaty, A., Shalaby, M. (2016). 'Circulating of is/1494-like strains of IBV in vaccinated commercial broiler flocks in Egypt during years 2012 to 2014', EKB Journal Management System, 62(2), pp. 29-35. doi: 10.21608/vmjg.2016.363155
SA, A., HA, H., A, A., Zanaty, A., Shalaby, M. Circulating of is/1494-like strains of IBV in vaccinated commercial broiler flocks in Egypt during years 2012 to 2014. EKB Journal Management System, 2016; 62(2): 29-35. doi: 10.21608/vmjg.2016.363155

Circulating of is/1494-like strains of IBV in vaccinated commercial broiler flocks in Egypt during years 2012 to 2014

Veterinary Medical Journal (Giza)
Volume 62, Issue 2, April 2016, Page 29-35  XML PDF (2.55 MB)
Document Type: Original Article
DOI: 10.21608/vmjg.2016.363155
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Authors
Abdelghany SA* 1; Hussein HA2; Arafa A3; Ali Zanaty3; MA Shalaby1
1Elwatania poultry grandparents, 6october- city stars - Giza, Egypt
2Department of virology, Faculty of veterinary Medicine, Cairo univveristy,12211 Giza, Egypt.
3National Laboratory for Veterinary Quality control on poultry Production , Animal Health Reserch Institute, P.O.Box 264- Dokki, Giza-12618, Egypt.
Abstract
In this study, the prevalence of IBV in Egyptian commercial Broiler farms were studied. Samples were collected from flocks in 9 Farms located in 5 governorates (Behira , Giza, Sharkia, Ismailia and Gharbia ) between years 2012 -2014. The birds showed severe respiratory signs with moderate number of chalky white droppings, feed consumption and body weight were markedly reduced. Severe rales with difficult respiration and mortalities were high and ranged from 20% to 25% . Flocks included in this study were vaccinated by classic Massachusetts strain similar commercial live (IB -H120 / MAS) vaccine. 8 out of 9 flocks were positive for IBV using Real time RT PCR. The 500-bp of the SI gene covering the hyper variable region-3 of 5 isolates were amplified and sequenced. Phylogenetic analysis based on that region revealed that these viruses closely related to Variant strains of IBV, The viruses were clustered into two groups, the first group included (port said F118-4/2013, Giza F299/2012) which were resemble to IS 1494 Variant 2 strains. The second group include (Behira F161/2012, Sharkia F552/2013 and Ismailia F187/2012) were vaccinal strains resemble D274 vaccine. Monitoring of evolving IBV strains is essential for improving the control of disease in Egypt.
Keywords
Infectious Bronchitis virus (IBV); S1 Gene; classic and variant; Egypt strains; Classic Vaccines
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