Effect of dimethyl-formamide and glycerol cryoprotectants on frozen stallion spermatozoa | ||||
| Veterinary Medical Journal (Giza) | ||||
| Volume 62, Issue 2, April 2016, Page 59-63 PDF (2.29 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/vmjg.2016.363158 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Aya Fadl* 1; El-Badry A2; Abou Ahmad M1; Ghallab M1 | ||||
| 1Theriogenology Dept., Faculty of veterinary medicine, Cairo university | ||||
| 2Artificial insemination and embryo transfer Dept., Anim. Reprod . Res. Instit. | ||||
| Abstract | ||||
| This study aimed to compare the efficiency of dimethyl-formamide (DMF), glycerol and their combination on freezability of Arabian stallion spermatozoa. Fourty semen samples were collected from four Arabian stallions, diluted with INRA-82 supplemented with 5% glycerol, 5% DMF or 2.5% glycerol+2.5% DME and subjected to cryopreservation. The results revealed the superiority of DMF vs. glycerol in terms of post-thaw motility (56.25 ± 0.89 vs. 46.50 ± 1.14%, respectively), viability index (167.75 ± 3.49 vs. 135.50 ± 4.49, respectively), survival rate (74.58 ± 1.03 vs. 61.59 ± 0.38%, respectively) and sperm acrosome integrity (78.83 ± 0.74 vs. 75.95 ± 0.74%, respectively). In conclusion, The protocols of Arabian stallion sperm cryopreservation could be enhanced using DMF as a cryoprotectant (CPA) used with INRA-82 extender. | ||||
| Keywords | ||||
| Arabian stallion- glycerol- dimethyl-formamide- cryoprotectant; freezing | ||||
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