A study on the Effect of Erythropoietin Treatment on Healing of Renal Damage in Male Albino Rats | ||||
| Bulletin of Egyptian Society for Physiological Sciences | ||||
| Article 2, Volume 29, Issue 1, June 2009, Page 11-24 PDF (258.09 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/besps.2009.36322 | ||||
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| Authors | ||||
| Romysa El-Sherbeny* 1; Mohamed Abd El-Rahman2 | ||||
| 1Physiology Department, Faculty of Medicine, Tanta University | ||||
| 2Pharmacology Department, Faculty of Medicine, Tanta University | ||||
| Abstract | ||||
| This study investigated the healing effect of erythropoietin treatment on renal damage in male albino rats. This work was carried out on 24 male albino rats, divided into four equal groups. Group (1) Control group: injected by 0.2ml saline intraperitonealy (2) Mercuric chloride (HgCl2) treated group: rats were injected intraperitonealy by single dose(3mg/kg) of HgCl2, group(3): Erythropoietin (Epo) treated group: rats were treated by intraperitoneal injection of Epo (1000u/kg) /day for 2 weeks, and group (4) HgCl2 and Epo treated group: rats were injected by single dose of HgCl2 and Epo for 2 weeks. At the end of experimental period, rats were sacrificed and blood samples were collected and sera were separated for estimation of serum levels of creatinine, urea, glutathione peroxidase, glutathione concentration, malondialdehyde and haematocrit value. The abdomen was dissected and kidney was excised and fixed in formalin for histopathological examination. The results showed in HgCl2 treated group, significant increase in serum creatinine, urea and malondialdehyde levels, and significant reduction in glutathione concentration, glutathione peroxidase and haematocrit value (HV) levels compared with control. Epo treated group showed significant reduction in serum levels of creatinine, and malondialdehyde, and significant increase in HV value levels, compared with the control. HgCl2 and Epo treated group showed, signification reduction in malondialdehyde, creatinine and urea and significant increase in glutathione concentration, glutathione peroxidase and HV compared with HgCl2 group. Histopathological examination showed necrosis of renal tubular epithelium and dilated proximal and distal tubules and wide Bowman's capsule in HgCl2 treated group. HgCl2 and Epo treated group showed improvement of renal tubular epithelium, mild dilatation of Bowman's capsule and bone marrow derived cells. It is concluded that, Epo treatment improved renal damage due to HgCl2 and promote healing of renal tissue, and it is recommended to be used in chronic renal disease. | ||||
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