Study on the Trophic Effect of Human Recombinant Erythropoietin on the Developing Small Bowel in Neonatal Rats | ||||
| Bulletin of Egyptian Society for Physiological Sciences | ||||
| Article 4, Volume 29, Issue 1, June 2009, Page 39-50 PDF (266.23 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/besps.2009.36324 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Romysa El-Sherbeny* ; Mahmoud El-Gharieb | ||||
| Physiology Dpartment, Faculty of Medicine, Tanta University | ||||
| Abstract | ||||
| The present work was done to study the trophic effect of human recombinant erythropoietin on the developing small bowel in neonatal rats. This study was carried out on 24 neonatal rats aged 4-5 days, weighing 40-60gm, and divided into 4 groups each containing 6 neonatal rats: Groups (1): was the control, group (2): administrated enteral human recombinant erythropoietin(Epo) through intragastric tube in a dose of 200 u/kg/day for one week, group (3): administrated enteral human recombinant Epo in a dose of 1000 u/kg/day through intragastric tube for one week, and group (4): administrated human recombinant Epo parenterally in a dose of 200u/kg/day for one week. At the end of the experiment, neonatal rats were weighed and blood was collected by cardiac puncture, sacrificed , dissected and small intestine was excised, length was measured and fixed in paraffin for histological examination. The results showed that administration of enteral recombinant Epo in dose of 200 and 1000 u/kg/day for a week caused significant increase in body weight and small bowel length , and non significant effect on haematocrit value or plasma erythropoietin concentration. The parenteral administration of Epo showed, significant increase in body weight, haematocrit value, plasma erythropoietin concentration and small bowel length. Histological examination showed, increased surface area of intestinal mucosa and increased length of the ilial villi.. It is concluded that, parenteral administration of human recombinant Epo has haematopoietic and trophic effects on the small bowel. Enteral administration of human recombinant Epo has a local trophic effect on small bowel, which is useful in treatment of infants suffering from defective absorption due to short bowel syndrome. | ||||
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