Early Transplantation of Human Umbalical Cord Blood Stem Cell Can Improve Engrafment and Liver Response to Carbon Tetra Chloride induced Cirrhosis in Mice | ||||
| Bulletin of Egyptian Society for Physiological Sciences | ||||
| Article 6, Volume 29, Issue 1, June 2009, Page 59-72 PDF (298.85 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/besps.2009.36327 | ||||
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| Authors | ||||
| Magdy El Barbary* 1; Howayda Abdel- All2; Mohamed Mohy Eldin3; Amal Yousef1; Sahar Greish1 | ||||
| 1Department of Physiology, Suez Canal University | ||||
| 2Department of Pathology, Suez Canal University | ||||
| 3Department of Internal Medicine, Suez Canal University | ||||
| Abstract | ||||
| Background: The potential use of UCB in the treatment of hepatic failure (a major problem worldwide) has been a research focus for several years now. Recent studies have identified UCB as a possible source of hepatic progenitor cells that can be differentiated into hepatocyte in vitro and in vivo and can ameliorate fibrosis. Objectives: the aim of this study was to investigate the hepatic response to transplantation of HUCB stem cells in CCl4 injured liver in mice, as regard liver function, histopathology and immunohistochemistry. Design: Experimental study. Setting: The stem cell unit in the Physiology Department and the animal house after cord blood collection in the Gynecology and Obstetric department, Faculty of Medicine, Suez Canal University. Materials and Methods: Hepatic fibrosis was induced by CCl4. HUCB stem cells were infused systemically through the tail vein immediately (group 1), and after one week of receiving CCl4 (group 2), Group 3 received only CCl4 (as a control group). Administration of CCl4 was continued for 10 weeks in G1, G2 and G3, while group 4 (as another control group) received only the solvent of CCl4 for 10 weeks. After that, blood from all groups was collected for assessment of liver function, then all mice were sacrified under general anesthesia and the liver was fixed and prepared for histopathological and immumohistochemical examination. Results: It was found that the level of alanine aminotransferase (ALT) in mice treated with stem cells after CCl4 administration was significantly lower while serum albumin was significantly higher compared to group 3 animals who received CCl4 without stem cells treatment (P= 0.001). Whereas serum total and direct bilirubin levels were similar among all groups. histopathological examination revealed that hepatic damage was less in the stem cells treated mice (G1 and G2) than in non treated group (as regards liver cell changes, portal tract inflammation, piecemeal necrosis, portal tract fibrosis and bridging fibrosis). However, liver inflammation and fibrosis were more in mice treated after one week than in immediately treated mice. Immumohistochemical examination, more importantly, IHC staining with monoclonal mouse anti-human hepatocyte revealed presence of human hepatocytes in injured mice liver which proved that the transplanted stem cells were transdifferentiated into hepatocyte. Conclusion: HUCB stem cells were transdifferentiated into hepatocyte when infused in mice injured liver and cause improvement in liver function test and liver histology. | ||||
| Keywords | ||||
| carbon tetrachloride (CCl4); Human Umbilical Cord Blood stem cells; liver fibrosis and monoclonal mouse anti-human hepatocyte | ||||
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