Evaluation of the Cyto-protective Effects of Erythropoietin on Contrast Induced Nephropathy in Rats | ||||
| Bulletin of Egyptian Society for Physiological Sciences | ||||
| Article 4, Volume 29, Issue 2, December 2009, Page 51-64 PDF (227.28 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/besps.2009.36352 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Moshira Rateb* 1; Maie Ismaiel2 | ||||
| 1Physiology Department, Faculty of Medicine, Cairo University | ||||
| 2Radiology Department, Helwan University Hospital | ||||
| Abstract | ||||
| Erythropoietin has long been used in renal failure patients for its hematopoeitic properties. It has recently been claimed to possess cytoprotective effects on many other cell lines including renal tubular cells .The aim of the present study was to assess the possible effects of erythropoietin on prevention of contrast induced renal failure in male rats. Methods: Thirty-two male albino rats were divided into four groups (n=8 each): control (C), contrast media (CM), erythropoietin (E) and rats treated with one single dose erythropoietin (4000 IU/Kg) IP 30 minutes before IV injection of the high osmolar contrast media diatrizoate (6 ml/kg) (E+CM). All the groups were subjected to dehydration for 72 hours before any injections were done. Hematological indices, kidney function parameters, oxidative stress markers, tumor necrosis factor alpha as a marker for inflammation and caspase 3 activity as a marker for apoptosis were measured after 72 hours of injections. Results: Injection of diatrizoate in dehydrated animals resulted in reduction of GFR, increased blood urea nitrogen and serum creatinine. It was associated with increased TNFα release and increased activation of caspase 3 as well as increased oxidative stress in kidney homogenates. Erythropoietin injection prior to the injection of the contrast medium significantly improved renal function parameters and was associated with a reduction in TNF alpha production, a reduction of caspase 3 activity and an increase in superoxide dismutase activity in the kidneys. Conclusion: Erythropoietin can infer protective effects to the kidneys against contrast induced nephropathy through mechanisms that are independent of its effect on the red cell mass. | ||||
| Keywords | ||||
| erythropoietin; contrast medium; renal failure; Oxidative Stress; apoptosis | ||||
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