Assessment of Galectins -1, -2, -3, and -8 Expression in Endometrial Carcinoma and Its Clinical Implications | ||||
| The Egyptian Journal of Fertility of Sterility | ||||
| Article 3, Volume 28, Issue 3 - Serial Number 11106352, May 2024, Page 11-25 PDF (6.54 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/egyfs.2024.363815 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Ahmed Sherif Abdel Hamid  1; Radwa M M Z Mohamed1; Hany Naeam1; Mahmoud M. Abdelfattah2; Amira A Ghonim2; Mohamed A M F Kortam1; Reham Helwa2
| ||||
| 1Department of Obstetrics and Gynecology, Faculty of Medicine, Ain Shams University, Cairo, Egypt. | ||||
| 2Molecular Cancer Biology group, Zoology Department, Faculty of Science, Ain Shams University, Cairo, Egypt. | ||||
| Abstract | ||||
| Background and aim: Galectins are a type of animal lectins that play a crucial role in regulating various cellular functions that can promote cancer progression including endometrial carcinoma. The aim of this study is to explore the expression of m RNA of galectins 1, 2, 3, and 8 in endometrial cancer and determine their relation to the extent of the disease. Methods: This case-control study was carried out at the Ain Shams University Maternity Hospital, as well as in the molecular biology laboratory located in the Zoology Department of the Faculty of Science at Ain Shams University. The study was conducted between October 2019 and January 2022. A total of 72 patients were scheduled for hysterectomy due to endometrial diseases. The study involved two groups. Group 1 comprised 60 women with endometrial malignancy, which includes atypical endometrial hyperplasia and/or endometrioid adenocarcinoma. Group 2 was the control group, which had 12 women with normal endometrial tissues. 58 tumor samples of endometrial pre-cancer and cancer lesions for mRNA expression using qRT-PCR. Results: The present study included 58 women with endometrial carcinoma, out of which almost 57% had endometrioid adenocarcinoma. Among them, 37 women were in FIGO stage I and II. The study found that LGALS1, LGALS2, LGALS3, and LGALS8 were significantly over-expressed in endometrial carcinoma patients as compared to the control group. However, the expression of these genes did not differ significantly when compared in different FIGO staging or based on the presence or absence of lymph node metastasis. There was also no significant difference in their expression when comparing patients with either endometrioid or non-endometrioid adenocarcinoma of the uterus. Conclusion: Our findings support the role of galectins in endometrial carcinogenesis, disease progression, and lymph node metastasis. Running title: Galectins in Endometrial Carcinoma | ||||
| Keywords | ||||
| Keywords: Endometrial carcinoma; galectins; mRNA; qRT-PCR; biomarkers; lymph node metastasis | ||||
|
Statistics Article View: 24 PDF Download: 13 |
||||
