Molecular Identification of Listeria Species from Some Processed Meat Products Using Specific Iap Gene and 16S rRNA Gene
Hassan, M., AbdAl-Aziz, S., Megahed, A., Abdel Rassoul, H., Mabrouk, Y. (2019). Molecular Identification of Listeria Species from Some Processed Meat Products Using Specific Iap Gene and 16S rRNA Gene. EKB Journal Management System, 40(APRIL- JUNE), 333-339. doi: 10.21608/asejaiqjsae.2019.36518
Mona M. Hassan; Samia A. AbdAl-Aziz; Asmaa M. Megahed; Hagar A. Abdel Rassoul; Yasser M. Mabrouk. "Molecular Identification of Listeria Species from Some Processed Meat Products Using Specific Iap Gene and 16S rRNA Gene". EKB Journal Management System, 40, APRIL- JUNE, 2019, 333-339. doi: 10.21608/asejaiqjsae.2019.36518
Hassan, M., AbdAl-Aziz, S., Megahed, A., Abdel Rassoul, H., Mabrouk, Y. (2019). 'Molecular Identification of Listeria Species from Some Processed Meat Products Using Specific Iap Gene and 16S rRNA Gene', EKB Journal Management System, 40(APRIL- JUNE), pp. 333-339. doi: 10.21608/asejaiqjsae.2019.36518
Hassan, M., AbdAl-Aziz, S., Megahed, A., Abdel Rassoul, H., Mabrouk, Y. Molecular Identification of Listeria Species from Some Processed Meat Products Using Specific Iap Gene and 16S rRNA Gene. EKB Journal Management System, 2019; 40(APRIL- JUNE): 333-339. doi: 10.21608/asejaiqjsae.2019.36518

Molecular Identification of Listeria Species from Some Processed Meat Products Using Specific Iap Gene and 16S rRNA Gene

Alexandria Science Exchange Journal
Article 11, Volume 40, APRIL- JUNE - Serial Number 2, April 2019, Page 333-339  XML PDF (405.96 K)
Document Type: Original Article
DOI: 10.21608/asejaiqjsae.2019.36518
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Authors
Mona M. Hassan email 1; Samia A. AbdAl-Aziz email 2; Asmaa M. Megahed* 1; Hagar A. Abdel Rassoul* 3; Yasser M. Mabrouk* 3
1Nucleic acids research department, Genetic Engineering and biotechnology Institute, City of Scientific research and Technological Applications, new Borg El Arab, Alexandria, Egypt
2Nucleic acids research department, Genetic Engineering and biotechnology Institute, City of Scientific research and Technological Applications, new Borg El Arab, Alexandria, Egypt
3Department of Genetics, Faculty of Agriculture (El-Shatby), Alexandria University, Alexandria, Egypt
Abstract
Three kinds of processed meat (Luncheon, Burger , Minced meat) were examined for the presence of Listeria species.Collected samples purchased from local supermarkets were from three different Egyptian companies. All the  bacterial isolates were cultured on specific media after pre-enrichment and enrichment cultures. The colonial morphology depends strongly up on the media used and the incubation conditions provided.  (Oxford Listeria Agar Base)was used as specific medium for Listeria. The bacterial  distribution of Listeria was, 2.22% in Beef burger, 13.6%. in luncheon and there was no bacterial growth in minced meat. In this study;  gram staining., morphological characterization, molecular identification using 16S rDNA  partial sequencing  and specific gene Iap gene  of Listeria were carried out.
Keywords
Listeria Species; specichic PCR
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