Genetic and Molecular Typing of Canine Parvovirus Strains Circulating in Symptomatic Dogs in Egypt | ||||
| Journal of Current Veterinary Research | ||||
| Article 10, Volume 1, Issue 1, June 2019, Page 75-85 PDF (1.17 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jcvr.2019.36556 | ||||
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| Authors | ||||
mohammed Nayel  1; Ahmed Zaghawa1; Mohamed Abualkhier2
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| 1Department of Animal Medicine and Infectious Diseases (Infectious Diseases), Faculty of Veterinary Medicine, University of Sadat City, Egypt | ||||
| 2Department of Virology, Faculty of Veterinary Medicine, University of Sadat City, Egypt | ||||
| Abstract | ||||
| Canine parvovirus (CPV) is considered one of the serious and problematic diseases in young puppies. It causes hemorrhagic enteritis and myocarditis in affected dogs. The aim of the present study was to detect CPV-2 in feces of clinically diseased puppies by polymerase chain reaction (PCR) followed by VP2 gene partial sequencing and molecular characterization of circulating strains in Egypt as well as studying some factors associated with the disease incidence. Forty fecal samples were collected from clinically suspected dogs with CPV-2. The clinical diagnosis was confirmed in 35 suspected clinical cases (87.5%) by PCR using common and specific primers sets for detection of (CPV-2, CPV-2a, CPV-2b, CPV-2c) validating that presumptive clinical diagnosis is practically dependable. Nucleotide sequencing of parvovirus (CPV-2) isolates showed that all the three antigenic types (2a, 2b, and 2c) are currently circulating in Egypt. | ||||
| Keywords | ||||
| CPV2; dogs; PCR; diarrhea | ||||
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