Mesenchymal stem cells promote the repair of chemotherapy-induced premature ovarian failure in female rats | ||||
| Benha Veterinary Medical Journal | ||||
| Volume 46, Issue 2, July 2024, Page 36-41 PDF (698.12 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bvmj.2024.283972.1814 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Heba Elsaied Sherif  1; Yakout Abdelfatah El- Senosi2; Samy Ali Hussein 2; Ehab Mahmoud Elzoghby3; Mohamed Ahmed Sobh4; Sara M. Farrag5; Basma Hamed Osman5
| ||||
| 1Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Benha University, Egypt | ||||
| 2Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Benha University, Egypt. | ||||
| 3Department of Histology, Faculty of Veterinary Medicine, Benha University, Egypt. | ||||
| 4Urology and Nephrology Center, Faculty of Medicine, Mansoura University, Egypt. | ||||
| 5Medical Experimental Research Center (MERC), Faculty of Medicine, Mansoura University, Egypt. | ||||
| Abstract | ||||
| Chemotherapeutic drugs, particularly alkylating cytotoxics such as cyclophosphamide (CYP) play a great job in inducing premature ovarian failure (POF). Hormone replacement therapy (HRT) is a widely used medication to improve hormone secretion. However, long-term HRT increases the risk of breast cancer and cardiovascular disease is concerning. Therefore, there is an urgent need to develop a safe and effective treatment for POF. Bone Marrow-(BM- MSCs) and Umbilical Cord -(UC- MSCs) derived stem cells (SCs) were isolated and identified from healthy rats. For POF induction, CYP (200mg/Kg b.wt) injected (i.p) into CYP, CYP+BMSCs, and CYP+UCMSCs followed by another dose (10mg/ b.wt, i.p) after one week. The normal control (NC) group were injected with saline. After two weeks of POF induction MSCs (1 × 106 cells/ml) of media were transplanted into POF rats through tail-vein. The effects of MSCs transplantation to POF were evaluated through sex hormones, iron, and complete blood count (CBC) analysis. POF-induced rats showed a significant increase in serum FSH and LH, with marked decrease in anti-Mullerian hormone (AMH) and estradiol (E2). Obvious increase in serum iron level observed in CYP group compared to the NC. Moreover, POF-induced rats displayed leucopenia, erythrocytopenia, and thrombocytopenia with a significant reduction in Hb, PCV, MCV, MCH, and MCHC compared to the NC. Administration of MSCs to CYP-injured rats significantly improved female reproductive hormones disturbances, iron, and hemogram. It was suggested that MSCs was a potential approach for improving sex hormones, iron, and hematological alterations, and potentially promoting the restoration of CYP-induced POF. | ||||
| Keywords | ||||
| Keywords: Complete blood count; Cyclophosphamide; Hormones; Mesenchymal stem cells; Premature ovarian failure | ||||
|
Statistics Article View: 190 PDF Download: 130 |
||||
