Antibacterial Potential of some Seaweeds Species to Combat Biofilm-producing Multi-drug Resistant Staphylococcus aureus of Nile Tilapia
Metwally, M., Ali, S., Khatab, I., El-Sayed, M. (2020). Antibacterial Potential of some Seaweeds Species to Combat Biofilm-producing Multi-drug Resistant Staphylococcus aureus of Nile Tilapia. EKB Journal Management System, 60(1), 9-24. doi: 10.21608/ejbo.2019.6829.1275
Metwally A. Metwally; Sameh Ali; Ismail Khatab; Mohamed Khairy El-Sayed. "Antibacterial Potential of some Seaweeds Species to Combat Biofilm-producing Multi-drug Resistant Staphylococcus aureus of Nile Tilapia". EKB Journal Management System, 60, 1, 2020, 9-24. doi: 10.21608/ejbo.2019.6829.1275
Metwally, M., Ali, S., Khatab, I., El-Sayed, M. (2020). 'Antibacterial Potential of some Seaweeds Species to Combat Biofilm-producing Multi-drug Resistant Staphylococcus aureus of Nile Tilapia', EKB Journal Management System, 60(1), pp. 9-24. doi: 10.21608/ejbo.2019.6829.1275
Metwally, M., Ali, S., Khatab, I., El-Sayed, M. Antibacterial Potential of some Seaweeds Species to Combat Biofilm-producing Multi-drug Resistant Staphylococcus aureus of Nile Tilapia. EKB Journal Management System, 2020; 60(1): 9-24. doi: 10.21608/ejbo.2019.6829.1275

Antibacterial Potential of some Seaweeds Species to Combat Biofilm-producing Multi-drug Resistant Staphylococcus aureus of Nile Tilapia

Egyptian Journal of Botany
Article 3, Volume 60, Issue 1, April 2020, Page 9-24  XML PDF (1.55 MB)
Document Type: Regular issue (Original Article)
DOI: 10.21608/ejbo.2019.6829.1275
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Authors
Metwally A. Metwally1; Sameh Ali email 1; Ismail Khatab2; Mohamed Khairy El-Sayed1
1Botany Department, Faculty of Science, Tanta University, Tanta 31527, Egypt
2Genetic Department, Faculty of Agriculture, Kafr El-Sheikh University, Kafr El-Sheik, Egypt
Abstract
THERE is an increasing worldwide demand for seafood due to the awareness of fish as an important protein source for a growing population. However, the diseases caused by multi-drug resistant (MDR) bacterial infections and the low survival rate of the fish represent significant challenges to fish farmers. The virulence and pathogenicity of bacteria are often enhanced when growing as a biofilm. Therefore, a variety of new antimicrobial drugs has attracted wide attention in treating fish pathogen bacteria. Herein, the efficiency of macroalgal extracts as an antibacterial agent against MDR Staphylococcus aureus bacteria isolated from Nile tilapia (Oreochromis niloticus) was studied. Out of 200 bacterial isolates, 50 strains were identified as S. aureus. Of these strains, 37 were MDR and showed a potential role in the production of virulence factors, including staphylokinase (70.2%), lecithinase (81%), protease (56.7%) and lipase (59.4%). Significant production of biofilm virulence factor by MDR S. aureus strains was also observed from the quantitative and qualitative analysis. Four algal species namely Jania rubens, Ulva lactuca, Sargassum vulgare, and Sargassum fusiforme were tested for their antibacterial activity against MDR S. aureus strains. Of those, S. vulgare diethyl ether extract showed the highest antibacterial. In addition, GC-MS analysis revealed 20 identified components in S. vulgare diethyl ether extract, in which Longifolene was dominant (16.5%). This study thus established the possibility of developing an antibacterial agent to combat developing MDR S. aureus and biofilm-related infections in Nile tilapia.
Keywords
Biofilm; Multi-drug resistance; Nile tilapia; Seaweeds; Staphylococcus aureus
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