Effect of Glutathione on Blood Pressure, Postnatal Lung Growth and Neonatal Outcome In A Rat Model of Pre-Eclampsia | ||||
| Bulletin of Egyptian Society for Physiological Sciences | ||||
| Article 8, Volume 28, Issue 1, June 2008, Page 91-106 PDF (316.67 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/besps.2008.36839 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Marwa Ahmed  1; Omayma Ahmed1; Asmaa Hassan2; Tarek Mostafa1
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| 1Department of Anatomy, Faculty of Medicine, Assiut University | ||||
| 2Department of Physiology, Faculty of Medicine, Assiut University | ||||
| Abstract | ||||
| Background: Pre-eclampsia is a human pregnancy-specific disorder that adversely affects the mother (by vascular dysfunction)and the fetus (by intrauterine growth restriction). A large portion of the prenatal mortality is consequently due to iatrogenic prematurity. Up to 15% of preterm births is a result of pre-eclampsia . The combination of hypertension plus proteinuria markedly increases the risk of prenatal morbidity and mortality over that of hypertension alone.Aim of the study:To elucidate the effect of addition of glutathione on lipid peroxidation products, blood pressure, proteinuria and neonatal outcome in a rat model of pre-eclampsia induced by NG-nitro-L-arginine-methyl ester (L-NAME), an inhibitor of nitric oxide synthase. Study Design: Adult pregnant rats (n =30) were allocated into three groups according to medication they received on day 17 from gestation to term. Rats were randomized into control group (n=10) and groups received L-NAME, 50 mg/day orally. (pre-eclampetic group, n = 10) and L-NAME + glutathione, 60mg/kg i.p. (treated group, n= 10) Blood levels of superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) were assessed .Mean systolic blood pressure (BP) (measured with a tail – cuff device), levels of proteinuria,total urine output ,pups birth weight and percentages of live and dead pups were recorded. Results: Mean systolic BP, plasma levels of SOD, CAT and MDA of pregnant rats were higher in pre-eclamptic group than in control group. Intreated group these plasma levels were lower than in pre-eclamptic group but still higher than those of control group. A positive correlation between systolic BPs on day 20 and SOD levels was recordedin both pre-eclamptic and treated groups. Birth weights of pups were higher in the control group than in the other groups. Pupsof treated group with glutathione had better survival rates than those of control and pre-eclamptic groups. The mean total body weight, lung weight, internal alveolar wall thickness and internal alveolar surface area of pups of pre-eclamptic group were significantly higher than those of control and treated groups. Histological changes: The lung specimens of pups of pre-eclamptic mothers showed irregular, collapsed, small alveoli. The lumnia of these alveoli contained oedematous fluid rich in erythrocytes. The interalveolar speta were relatively thickened and more cellular than control. The treated group showed more or less the same morphological findings as the control group.Conclusion:In this rat model of pre-eclampsia, hypertension, proteinuria, oxidative stress of pregnant rats, a high neonatal death rate and pathological changes of lungs structure of pups, are reversed by exogenous antioxidant use. | ||||
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