A Comparison between Some Methods Used for Extraction and Estimation of Testosterone Hormone in Meat
Hassan, O., Tag El -Din, H., Ahmed, N., Salem, G. (2014). A Comparison between Some Methods Used for Extraction and Estimation of Testosterone Hormone in Meat. EKB Journal Management System, 60(1), 111-120. doi: 10.21608/vmjg.2014.368819
Omaima Hassan; Hannan Tag El -Din; Nashwa Ahmed; Ghada Salem. "A Comparison between Some Methods Used for Extraction and Estimation of Testosterone Hormone in Meat". EKB Journal Management System, 60, 1, 2014, 111-120. doi: 10.21608/vmjg.2014.368819
Hassan, O., Tag El -Din, H., Ahmed, N., Salem, G. (2014). 'A Comparison between Some Methods Used for Extraction and Estimation of Testosterone Hormone in Meat', EKB Journal Management System, 60(1), pp. 111-120. doi: 10.21608/vmjg.2014.368819
Hassan, O., Tag El -Din, H., Ahmed, N., Salem, G. A Comparison between Some Methods Used for Extraction and Estimation of Testosterone Hormone in Meat. EKB Journal Management System, 2014; 60(1): 111-120. doi: 10.21608/vmjg.2014.368819

A Comparison between Some Methods Used for Extraction and Estimation of Testosterone Hormone in Meat

Veterinary Medical Journal (Giza)
Volume 60, Issue 1, January 2014, Page 111-120  XML PDF (4.16 MB)
Document Type: Original Article
DOI: 10.21608/vmjg.2014.368819
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Authors
Omaima Hassan1; Hannan Tag El -Din2; Nashwa Ahmed1; Ghada Salem email 3
1chemistry Department, Animal Health Research Institute, Agriculture Research Center, Dokki, Giza, Egypt.
2chemistry Department, Animal Health Research Institute, Agriculture Research Center, Dokki, Giza, Egypt.
3Food Hygiene Department, Animal Health Research Institute, Agriculture Research Center, Dokki, Giza, Egypt.
Abstract
The increased public concern and the launch of legislative Strategies within the framework of method validation and risk evaluation induced the need for the development of highly sensitive and specific analytical methods for the determination of steroid hormones in edible matrices. In this study, three extraction techniques of testosterone residues are tested, the manufacturer procedure described by the ELISA kits for testosterone in tissues, the method stated by Umberger et al. (1958) without or with cleanup. Extraction followed by estimation of testosterone residue by ELISA kits either for tissue or serum. Fifty imported meat samples were collected randomly from markets; each was subjected to six tested treatments. The cost of chemicals, materials and kits of each treatment was computed to be compared economically. The obtained results indicated that, there is a significant testosterone residue increase in the extraction techniques stated by Umberger et al., (1958). Also, clean up procedure has no significant effect. Moreover, there is no significant difference between commercial ELISA kits for tissues and that for serum the most expensive treatment was that described by manufacturer of r- Biopharm Kit. On the other hand the cheapest treatment was the extraction according to Umberger et al., (1958) followed by detecting testosterone residues using commercial kit for estimation of testosterone in serum. Taking into consideration both significance and economical points of view it is recommended to use Umberger et al., (1958) for extraction of meat testosterone and commercial ELISA kit for serum to detect testosterone residue in the extract.
 
Keywords
ELISA; Meat; Serum; Testosterone
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