Insights into the role of fibroblasts in non-small cell lung cancer cell lines progression as a potential therapeutic target | ||||
| Journal of Advanced Pharmacy Research | ||||
| Articles in Press, Accepted Manuscript, Available Online from 23 July 2024 | ||||
| Document Type: Research Article | ||||
| DOI: 10.21608/aprh.2024.295637.1275 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Nouran Ebid1; Marwa Sharaky2; Abeer Elkhoely  3; Engy El Morsy3; Sherif Saad2
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| 1Pharmacology & Toxicology Department, Faculty of Pharmacy, Egyptian Russian University, Badr City, Cairo, Egypt | ||||
| 2Pharmacology & Experimental Oncology unit, Cancer Biology department, National Cancer Institue, Cairo University, Cairo, Egypt | ||||
| 3Pharmacology & Toxicology Department, Faculty of Pharmacy, Helwan University, Cairo, Egypt | ||||
| Abstract | ||||
| Objectives Fibroblasts are one of the most abundant cells in the tumor microenvironment, often playing a dual role in the cancer progression. Normal fibroblasts can have tumor suppressive properties at the initial stages of cancer, but upon stimulation by cancer cells they acquire tumor promoting qualities by transitioning into Cancer associated fibroblasts. The present study aimed to investigate the cross-talk that interplays between fibroblasts and cancer cells and its impact on cancer progression, metastasis and apoptosis reflected by the expression of epidermal growth factor receptor (EGFR) and its ligands, fibroblast growth factor-3 (FGF-3) and its receptor, angiogenic markers, vascular endothelial growth factor (VEGF) and matrix metalloproteinases, and various caspases. Methods Fibroblast and cancer cell co-culture was done directly and indirectly through conditioned media. ELISA technique was used to assess the concentration of the studied biochemical markers. Results Our results reveled an upregulation in the aforementioned parameters in A549 cell lines upon co-culture with fibroblasts, our study also revealed a temporal aspect, as their expression increased overtime. Our study also revealed that the markers’ overexpression was more pronounced in direct co-culture method, implicating the significance of direct cell-cell interaction Conclusion In conclusion, our study provides insight on the complex nature of the interaction between fibroblasts and cancer cells highlighting the need for further studies. | ||||
| Keywords | ||||
| Fibroblasts; cancer; co-culture; EGFR; FGF-3 | ||||
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