MOLECULAR CHARACTERIZATION OF THREE EGYPTIAN INFECTIOUS BRONCHITIS VIRUS ISOLATES | ||||
Veterinary Medical Journal (Giza) | ||||
Volume 50, Issue 4, October 2002, Page 1053-1064 PDF (3.18 MB) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/vmjg.2002.369385 | ||||
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Authors | ||||
H MADBOULY* 1; A ABDEL-MONEIM1; J JR2; B LADMAN2; NANCY ROFAEL3 | ||||
1Virology Department, Faculty of Veterinary Medicine, Cairo University, Beni-Seuf Branch, Egypt. | ||||
2Department of Animal and Food Sciences, University of Delaware, Newark, DE 19717 | ||||
3Serum and Vaccine Research Institute, Abbasia, Egypt | ||||
Abstract | ||||
Three field infectious bronchitis virus (IBV) strains isolated from different governorates in Egypt were examined molecularly using reverse transcriptase polymerase chain reaction (RT- PCR) and S1 partial gene sequence. RT-PCR using universal primers was positive for the three isolates. S-1 sequence analysis was successfully performed with two isolates only and failed with the third one. SI analysis of the two isolates revealed that one isolate was identical (100% aminoacid and nucleotide similarities) to Egypt/BeniSeuf/01 of a newly discovered genotype. The other isolate showed close similarity to Dutch variants D274 (98% aminoacid similarity and 99.4 %nucleotide similarity) and D3896 (94% aminoacid similarity and 97.8 %nucleotide similarity). Both isolates showed considerable high divergence to H120 (29.8% amino acid divergence and 25.8% nucleotide divergence for isolate 1 and 26.9% amino acid divergence and 23.9% nucleotide divergence for isolate 2) and M41 (30.4% amino acid divergence and 25% nucleotide divergence for isolate 1 and 28.0% amino acid divergence and 22.6% nucleotide divergence for isolate 2) vaccines. | ||||
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