MOLECULAR CHARACTERIZATION OF THREE EGYPTIAN INFECTIOUS BRONCHITIS VIRUS ISOLATES
MADBOULY, H., ABDEL-MONEIM, A., JR, J., LADMAN, B., ROFAEL, N. (2002). MOLECULAR CHARACTERIZATION OF THREE EGYPTIAN INFECTIOUS BRONCHITIS VIRUS ISOLATES. EKB Journal Management System, 50(4), 1053-1064. doi: 10.21608/vmjg.2002.369385
H MADBOULY; A ABDEL-MONEIM; J JR; B LADMAN; NANCY ROFAEL. "MOLECULAR CHARACTERIZATION OF THREE EGYPTIAN INFECTIOUS BRONCHITIS VIRUS ISOLATES". EKB Journal Management System, 50, 4, 2002, 1053-1064. doi: 10.21608/vmjg.2002.369385
MADBOULY, H., ABDEL-MONEIM, A., JR, J., LADMAN, B., ROFAEL, N. (2002). 'MOLECULAR CHARACTERIZATION OF THREE EGYPTIAN INFECTIOUS BRONCHITIS VIRUS ISOLATES', EKB Journal Management System, 50(4), pp. 1053-1064. doi: 10.21608/vmjg.2002.369385
MADBOULY, H., ABDEL-MONEIM, A., JR, J., LADMAN, B., ROFAEL, N. MOLECULAR CHARACTERIZATION OF THREE EGYPTIAN INFECTIOUS BRONCHITIS VIRUS ISOLATES. EKB Journal Management System, 2002; 50(4): 1053-1064. doi: 10.21608/vmjg.2002.369385

MOLECULAR CHARACTERIZATION OF THREE EGYPTIAN INFECTIOUS BRONCHITIS VIRUS ISOLATES

Veterinary Medical Journal (Giza)
Volume 50, Issue 4, October 2002, Page 1053-1064  XML PDF (3.18 MB)
Document Type: Original Article
DOI: 10.21608/vmjg.2002.369385
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Authors
H MADBOULY* 1; A ABDEL-MONEIM1; J JR2; B LADMAN2; NANCY ROFAEL3
1Virology Department, Faculty of Veterinary Medicine, Cairo University, Beni-Seuf Branch, Egypt.
2Department of Animal and Food Sciences, University of Delaware, Newark, DE 19717
3Serum and Vaccine Research Institute, Abbasia, Egypt
Abstract
Three field infectious bronchitis virus (IBV) strains isolated from different governorates in Egypt were examined molecularly using reverse transcriptase polymerase chain reaction (RT- PCR) and S1 partial gene sequence. RT-PCR using universal primers was positive for the three isolates. S-1 sequence analysis was successfully performed with two isolates only and failed with the third one. SI analysis of the two isolates revealed that one isolate was identical (100% aminoacid and nucleotide similarities) to Egypt/BeniSeuf/01 of a newly discovered genotype. The other isolate showed close similarity to Dutch variants D274 (98% aminoacid similarity and 99.4 %nucleotide similarity) and D3896 (94% aminoacid similarity and 97.8 %nucleotide similarity). Both isolates showed considerable high divergence to H120 (29.8% amino acid divergence and 25.8% nucleotide divergence for isolate 1 and 26.9% amino acid divergence and 23.9% nucleotide divergence for isolate 2) and M41 (30.4% amino acid divergence and 25% nucleotide divergence for isolate 1 and 28.0% amino acid divergence and 22.6% nucleotide divergence for isolate 2) vaccines.
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