OPTIMIZATION OF EXTRACTION PROCESS TO MAXIMIZE PHENOLIC CONTENT, FLAVONOID CONTENT, AND ANTIOXIDANT ACTIVITY OF PROSOPIS FARCTA USING RESPONSE SURFACE METHODOLOGY
Hamwi, M., Nayal, R., Abajy, M. (2024). OPTIMIZATION OF EXTRACTION PROCESS TO MAXIMIZE PHENOLIC CONTENT, FLAVONOID CONTENT, AND ANTIOXIDANT ACTIVITY OF PROSOPIS FARCTA USING RESPONSE SURFACE METHODOLOGY. EKB Journal Management System, 47(2), 883-898. doi: 10.21608/bfsa.2024.287313.2110
Mona Hamwi; Ream Nayal; Mohammad Yaser Abajy. "OPTIMIZATION OF EXTRACTION PROCESS TO MAXIMIZE PHENOLIC CONTENT, FLAVONOID CONTENT, AND ANTIOXIDANT ACTIVITY OF PROSOPIS FARCTA USING RESPONSE SURFACE METHODOLOGY". EKB Journal Management System, 47, 2, 2024, 883-898. doi: 10.21608/bfsa.2024.287313.2110
Hamwi, M., Nayal, R., Abajy, M. (2024). 'OPTIMIZATION OF EXTRACTION PROCESS TO MAXIMIZE PHENOLIC CONTENT, FLAVONOID CONTENT, AND ANTIOXIDANT ACTIVITY OF PROSOPIS FARCTA USING RESPONSE SURFACE METHODOLOGY', EKB Journal Management System, 47(2), pp. 883-898. doi: 10.21608/bfsa.2024.287313.2110
Hamwi, M., Nayal, R., Abajy, M. OPTIMIZATION OF EXTRACTION PROCESS TO MAXIMIZE PHENOLIC CONTENT, FLAVONOID CONTENT, AND ANTIOXIDANT ACTIVITY OF PROSOPIS FARCTA USING RESPONSE SURFACE METHODOLOGY. EKB Journal Management System, 2024; 47(2): 883-898. doi: 10.21608/bfsa.2024.287313.2110

OPTIMIZATION OF EXTRACTION PROCESS TO MAXIMIZE PHENOLIC CONTENT, FLAVONOID CONTENT, AND ANTIOXIDANT ACTIVITY OF PROSOPIS FARCTA USING RESPONSE SURFACE METHODOLOGY

Bulletin of Pharmaceutical Sciences Assiut University
Volume 47, Issue 2, December 2024, Page 883-898  XML PDF (982.79 K)
Document Type: Original Article
DOI: 10.21608/bfsa.2024.287313.2110
View on SCiNiTO View on SCiNiTO
Authors
Mona Hamwi email 1; Ream Nayalorcid 1; Mohammad Yaser Abajyorcid 2
1Department of Pharmacognosy, Faculty of Pharmacy, University of Aleppo, Aleppo, Syria
2Department of Biochemistry and Microbiology, Faculty of Pharmacy, University of Aleppo, Aleppo, Syria.
Abstract
Prosopis farcta (Banks et Sol.) Eig., is a widely distributed plant, that is rich in health benefits components. This study aims to optimize the ultrasonic-assisted extraction parameters of P. farcta leaves in order to maximize total phenolic content (TPC), total flavonoid content (TFC), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, using response surface methodology (RSM). A Box-Behnken design (BBD) with three levels and three variables was employed, the independent variables were extraction temperature (20, 40 and 60°C), extraction time (20, 40 and 60 minutes) and ethanol concentration (20, 45 and 70%). Results show that all three extraction parameters have great effects on the TPC, TFC, and DPPH scavenging values, the optimal conditions were (extraction temperature: 53.93°C, extraction time: 51.11 minutes and ethanol concentration: 65.45%), under these conditions, the experimental results were (TPC: 109.89 mg GAE/g DW, TFC: 11.287 mg RE/g DW and DPPH: 37.503%), these results are matching well with the theoretical predicted values which proves that RSM models were accurate and reliable. Strong correlations were found between TPC, TFC, and DPPH scavenging activity. This study revealed the importance of P. farcta as a natural source of antioxidants, and highlighted the optimal extraction conditions that can be effectively employed for maximizing production of natural antioxidants from P. farcta leaves.
Keywords
Prosopis farcta; antioxidant; ultrasonic-assisted extraction; optimization; response surface methodology (RSM)
Statistics
Article View: 436
PDF Download: 292