Histophysiological Evaluation of the Effect of Alpha-Tocopherol and Dimethyl Diphenyl Bicarboxylate on Arsenic-Induced Hepatotoxicity in Adult Male Albino Rats | ||||
| Bulletin of Egyptian Society for Physiological Sciences | ||||
| Article 17, Volume 28, Issue 1, June 2008, Page 225-252 PDF (1.35 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/besps.2008.36956 | ||||
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| Authors | ||||
| Ahmed Elshennawy* 1; Mahmoud El-Gharieb2; Mohammad Hashem3 | ||||
| 1Department of Histology, Faculty of Medicine, El-Minya University | ||||
| 2Department of Physiology, Faculty of Medicine, Tanta University | ||||
| 3Department of Forensic & Toxicology, Faculty of Medicine, El-Minya University, Egypt | ||||
| Abstract | ||||
| Arsenic, a widely studied medicinal and toxicological element, is known to induce oxidative stress and damage to cells. The present study was aimed at to assess the effect of vitamin E (alpha-tocopherol) and/or DDB (dimethyl diphenyl bicarboxylate) with or without the chelator DMSA (Meso 2,3-dimercaptosuccinic acid) on arsenic-induced hepatotoxicity. Fifty four adult malealbino rats were divided into nine groups. Group I was the control and received only intraperitoneal injection normal saline 2 times per week for two weeks. Group II was injected with sodium arsenite in normal saline 2 times /week for 2 weeks. Group III was injected with sodium arsenite and received oral DMSA daily for 2 weeks. Group IV was injected with sodium arsenite and received oral vitamin E (alpha –tocopherol) daily for 2 weeks. Group V was injected with sodium arsenite and received oral DDB daily for 2 weeks. Group VI received sodium arsenite, vitamin E and DDB for 2 weeks. Group VII received sodium arsenite, DMSA and vitamin E for 2 weeks. Group VIII received sodium arsenite, DMSA and DDB for 2 weeks. Group IX received sodium arsenite, DMSA, vitamin E and DDB for 2 weeks. Physiological and biochemical parameters were undertaken to measure Alanine Amino-Transferase (ALT), Aspartate Amino-Transferase (AST), Malondialdehyde (MDA), reduced Glutathione (GSH) and Glutathione Peroxidase (GPx). Histochemical and histpathological parameters were also undertaken to assess the structural-functional mirror changes. The results of this study showed that vitamin E and DDB were almost similar in their antioxidant hepatoprotective effects with stronger inhibiting action of DDB to lipid peroxidation while the greatest effect was achieved by their combination witha chelating agent like DMSA with restoration of almost the normal hepatic histology. | ||||
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