DYSREGULATION OF EZRIN EXPRESSION AND PHOSPHORYLATION IN UTERINE LEIOMYOMA | ||
| Bulletin of Pharmaceutical Sciences Assiut University | ||
| Volume 47, Issue 2, December 2024, Pages 1327-1339 PDF (614.02 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bfsa.2024.299419.2175 | ||
| Authors | ||
| khaled Mohammed Hassan yousef* 1; Abdel-Raheim Mohamed Meki2, 3; Ayat Abdel-Rahman Sayed2; Muhammad Maghraby4; Tahia H Saleem2 | ||
| 1Department of Biochemistry, Faculty of Pharmacy, South Valley University, Qena, Egypt | ||
| 2Department of Medical Biochemistry, Faculty of Medicine, Assiut University, Egypt | ||
| 3Department of Biochemistry, Faculty of Pharmacy, Sphinx University, New Assiut, Egypt | ||
| 4Medical student, Faculty of Medicine, Assiut university, Egypt | ||
| Abstract | ||
| Uterine Leiomyoma (UL) is a major source of gynecologic and reproductive dysfunction in females of reproductive age. They are highly fibrotic tumors with evident disturbance of the mechano-transduction molecules. Ezrin acts as a dynamic linkage between the cytoskeleton and the membrane-associated proteins known to be dysregulated in tumorigenesis. Aim of the study: The current study aimed to profile the expression of ezrin, phospho-ezrin, and their upstream effector molecules; RhoA and ROCK1 in UL, their adjacent tissues, and control myometrium. Materials and Methods: Ezrin, phospho-ezrin, RhoA and ROCK1 mRNA and proteins were assayed in the tissue lysate of fibroid, and adjacent myometrium of 43 pre-menopausal women with symptomatic UL and 18 non-fibroid myometrium as a control group. Results: Tissue levels of ezrin, phospho-ezrin, RhoA and Rock in fibroid and adjacent tissues were higher compared to the control tissue. Moreover, the levels of phospho-ezrin and total RhoA proteins showed higher levels in fibroid compared to adjacent tissues. Phospho-ezrin and ROCK1 mRNA correlated positively with both uterine and fibroid size. Conclusion: Ezrin, phospho-ezrin, and RhoA-ROCK upregulation in leiomyoma and its adjacent myometrium signify the central role of ezrin and its upstream and downstream effector on the pathogenesis and progression of UL. | ||
| Keywords | ||
| Ezrin; phospho-ezrin; RhoA; ROCK1; leiomyoma | ||
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