Cryopreservation of rare ejaculate and testicular spermatozoa, a novel simple technique; the culture dish slice freezing: A pilot study.
Amer, M., Fikry, I., Aldsoqy, A., Attia, I., Mostafa, M., Fakhry, E. (2024). Cryopreservation of rare ejaculate and testicular spermatozoa, a novel simple technique; the culture dish slice freezing: A pilot study.. EKB Journal Management System, 1(2), 87-93. doi: 10.21608/jrme.2024.267502.1011
Medhat Amer; Ihab Fikry; Alshaimaa Aldsoqy; Iman Attia; Mohammed Mostafa; Emad Fakhry. "Cryopreservation of rare ejaculate and testicular spermatozoa, a novel simple technique; the culture dish slice freezing: A pilot study.". EKB Journal Management System, 1, 2, 2024, 87-93. doi: 10.21608/jrme.2024.267502.1011
Amer, M., Fikry, I., Aldsoqy, A., Attia, I., Mostafa, M., Fakhry, E. (2024). 'Cryopreservation of rare ejaculate and testicular spermatozoa, a novel simple technique; the culture dish slice freezing: A pilot study.', EKB Journal Management System, 1(2), pp. 87-93. doi: 10.21608/jrme.2024.267502.1011
Amer, M., Fikry, I., Aldsoqy, A., Attia, I., Mostafa, M., Fakhry, E. Cryopreservation of rare ejaculate and testicular spermatozoa, a novel simple technique; the culture dish slice freezing: A pilot study.. EKB Journal Management System, 2024; 1(2): 87-93. doi: 10.21608/jrme.2024.267502.1011

Cryopreservation of rare ejaculate and testicular spermatozoa, a novel simple technique; the culture dish slice freezing: A pilot study.

Journal of Reproductive Medicine and Embryology
Article 3, Volume 1, Issue 2, July 2024, Page 87-93  XML PDF (439.36 K)
Document Type: Clinical Trial
DOI: 10.21608/jrme.2024.267502.1011
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Authors
Medhat Amer1, 2, 3; Ihab Fikry2; Alshaimaa Aldsoqy2; Iman Attia2; Mohammed Mostafa2; Emad Fakhry email orcid 2
1Andrology Department, Faculty of Medicine, Cairo University, Egypt.
2Adam International Hospital, Cairo, Egypt.
3Egyptian Foundation of Reproductive Medicine and Embryology (EFRE).
Abstract
Abstract:
Objective: The ability to cryopreserve small numbers of spermatozoa retrieved by testicular sperm extraction (TESE) in males having nonobstructive azoospermia avoids the requirement for repeated surgery and promotes the preservation of fertility. The same applies to men with cryptozoospermia who may show few sperms only after concentrating their semen samples. Many techniques were proposed for freezing rare motile spermatozoa. In this pilot study we propose a new simple and safe technique that requires no specific commercial tools.
Methodology: A new method is suggested for cryopreserving rare human spermatozoa using the culture dish slice.
Results: Four cases were included in this pilot study. A total of 20 motile ejaculate sperms and 20 motile testicular sperms were frozen using the culture dish slice technique. Sperm retrieval, retrieval of motility and retrieval of usable sperms for ICSI were (100%, 25% and 70% respectively) for ejaculate sperms and (100%, 10% and 60% respectively) for testicular sperms.
Conclusion: The culture dish slice technique would represent an easy, non-expensive method for rare sperm cryopreservation that necessitates no special commercial devices.
Keywords
Sperm; cryopreservation; functional azoospermia; testicular
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