The Effect of Using Different Additives to the Media for Production of Highly Viable Rev-1 Vaccine
Ghazi, N., Hussein, K. (2014). The Effect of Using Different Additives to the Media for Production of Highly Viable Rev-1 Vaccine. EKB Journal Management System, 8(1), 7-11. doi: 10.21608/jcvr.2014.37167
Nabila Ghazi; K.E Hussein. "The Effect of Using Different Additives to the Media for Production of Highly Viable Rev-1 Vaccine". EKB Journal Management System, 8, 1, 2014, 7-11. doi: 10.21608/jcvr.2014.37167
Ghazi, N., Hussein, K. (2014). 'The Effect of Using Different Additives to the Media for Production of Highly Viable Rev-1 Vaccine', EKB Journal Management System, 8(1), pp. 7-11. doi: 10.21608/jcvr.2014.37167
Ghazi, N., Hussein, K. The Effect of Using Different Additives to the Media for Production of Highly Viable Rev-1 Vaccine. EKB Journal Management System, 2014; 8(1): 7-11. doi: 10.21608/jcvr.2014.37167

The Effect of Using Different Additives to the Media for Production of Highly Viable Rev-1 Vaccine

Journal of Current Veterinary Research
Article 2, Volume 8, Issue 1, 2014, Page 7-11  XML
Document Type: Original Article
DOI: 10.21608/jcvr.2014.37167
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Authors
Nabila Ghazi; K.E Hussein
Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo, Egypt
Abstract
Four media with different additives were prepared and used for propagation of Brucella melitensis rev 1 strain for vaccine production and compared with the currently used serum trypticase soya agar medium. All the used nutrient additives gave good results with different degrees, It was found that medium with yeast extract and protose -ME was the best in growing of the strain as measured by packed cell volume .Vaccines prepared from the strain grown on those media showed no different results in potency of Guinea pigs. So this study recommends using yeast extract and protose -ME as additives to give highest packed cell volume without changing strain character.
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