Isolation and Identification of Clostridium butyricum as a Causative Agent of Necrotizing Enterocolitis in Rabbits
El-Helw, H., Abdella,, Y., El-Sergany, E. (2014). Isolation and Identification of Clostridium butyricum as a Causative Agent of Necrotizing Enterocolitis in Rabbits. EKB Journal Management System, 8(1), 23-31. doi: 10.21608/jcvr.2014.37171
H. A. El-Helw; Y.A. Abdella,; Elham El-Sergany. "Isolation and Identification of Clostridium butyricum as a Causative Agent of Necrotizing Enterocolitis in Rabbits". EKB Journal Management System, 8, 1, 2014, 23-31. doi: 10.21608/jcvr.2014.37171
El-Helw, H., Abdella,, Y., El-Sergany, E. (2014). 'Isolation and Identification of Clostridium butyricum as a Causative Agent of Necrotizing Enterocolitis in Rabbits', EKB Journal Management System, 8(1), pp. 23-31. doi: 10.21608/jcvr.2014.37171
El-Helw, H., Abdella,, Y., El-Sergany, E. Isolation and Identification of Clostridium butyricum as a Causative Agent of Necrotizing Enterocolitis in Rabbits. EKB Journal Management System, 2014; 8(1): 23-31. doi: 10.21608/jcvr.2014.37171

Isolation and Identification of Clostridium butyricum as a Causative Agent of Necrotizing Enterocolitis in Rabbits

Journal of Current Veterinary Research
Article 4, Volume 8, Issue 1, 2014, Page 23-31  XML
Document Type: Original Article
DOI: 10.21608/jcvr.2014.37171
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Authors
H. A. El-Helw; Y.A. Abdella,; Elham El-Sergany
Veterinary Serum and Vaccine Research Institute, Egypt
Abstract
Clostridium butyricum is one of the members of genus Clostridia causing necrotizing enterocolitis in rabbit that characterized by intestinal damage (congestion and patchy hemorrhage in intestinal mucosa) and enlargement of caecum (cecitis). In this study, out of 100 rabbits suffered from severe abdominal distension and diarrhea, 34 isolates suspect to be C. butyricum according to their culture characters and microscopical examinations. Pathogenicity test was performed on susceptible rabbits giving the same symptoms and the same strain was re-isolated again. Suspected isolates were identified by detection of 16S rDNA gene using Polymerase Chain Reaction, sequencing of PCR product and comparing sequencing with Gen Bank and that resulting in 98% homogenicity to C. butyricum. These results confirmed that isolated strain is C. butyricum and could be used in further studies in preparation of vaccine for controlling necrotizing enterocolitis in rabbits.
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