Studies on clinical and subclinical mastitis in Menoufia Governate with application of PCR for diagnosis | ||||
| Journal of Current Veterinary Research | ||||
| Article 9, Volume 9, Issue 1, 2015, Page 78-84 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jcvr.2015.37190 | ||||
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| Authors | ||||
Walid Mousa  ; Ahmed Zaghawa; Mohammed Nayel
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| Department of Animal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, University of Sadat City, Menoufia 32511, Egypt. | ||||
| Abstract | ||||
| Bovine mastitis is a serious problem in the dairy herds all over the world. In this study 105 mastitic milk samples were collected from small holder’s cows for bacteriological and molecular diagnosis. The prevalence of clinical and subclinical mastitis were 20.5% and 32% respectively which were detected by clinical examination and California Mastitis Test respectively. Bacteriological results revealed that Staphylococcus aureus was the most common isolated bacteria from both clinical and subclinical mastitis. Streptococcus species, Pseudomonas species, E. coli and Enterobacter species were also isolated. Application of multiplex PCR was effective in identification of bacteria causing mastitis directly from milk samples and from extracted DNA of bacteria | ||||
| Keywords | ||||
| mastitis; prevalence; Staphylococcus aureus; Multiplex PCR | ||||
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