IN-VITRO STUDY ON THE EFFECT OF CHITOSAN, AND CHITOSAN NANOPARTICLES ON THE VIABILITY AND ULTRASTRUCTURE OF BLASTOCYSTIS SPECIES
EZZ EL DINE, S., AL-ANTABLY, A., EL BAHY, M., EL AKAD, D., RIZK, E. (2024). IN-VITRO STUDY ON THE EFFECT OF CHITOSAN, AND CHITOSAN NANOPARTICLES ON THE VIABILITY AND ULTRASTRUCTURE OF BLASTOCYSTIS SPECIES. EKB Journal Management System, 54(2), 283-294. doi: 10.21608/jesp.2024.373531
SARAH IBRAHIM MOHAMED EZZ EL DINE; ABEER SAID ABD EL-GHANY AL-ANTABLY; MOHAMED M. EL BAHY; DINA MOHAMED HAMDY EL AKAD; ENAS M. A. RIZK. "IN-VITRO STUDY ON THE EFFECT OF CHITOSAN, AND CHITOSAN NANOPARTICLES ON THE VIABILITY AND ULTRASTRUCTURE OF BLASTOCYSTIS SPECIES". EKB Journal Management System, 54, 2, 2024, 283-294. doi: 10.21608/jesp.2024.373531
EZZ EL DINE, S., AL-ANTABLY, A., EL BAHY, M., EL AKAD, D., RIZK, E. (2024). 'IN-VITRO STUDY ON THE EFFECT OF CHITOSAN, AND CHITOSAN NANOPARTICLES ON THE VIABILITY AND ULTRASTRUCTURE OF BLASTOCYSTIS SPECIES', EKB Journal Management System, 54(2), pp. 283-294. doi: 10.21608/jesp.2024.373531
EZZ EL DINE, S., AL-ANTABLY, A., EL BAHY, M., EL AKAD, D., RIZK, E. IN-VITRO STUDY ON THE EFFECT OF CHITOSAN, AND CHITOSAN NANOPARTICLES ON THE VIABILITY AND ULTRASTRUCTURE OF BLASTOCYSTIS SPECIES. EKB Journal Management System, 2024; 54(2): 283-294. doi: 10.21608/jesp.2024.373531

IN-VITRO STUDY ON THE EFFECT OF CHITOSAN, AND CHITOSAN NANOPARTICLES ON THE VIABILITY AND ULTRASTRUCTURE OF BLASTOCYSTIS SPECIES

Journal of the Egyptian Society of Parasitology
Article 14, Volume 54, Issue 2, August 2024, Page 283-294  XML PDF (2.75 MB)
Document Type: Original Article
DOI: 10.21608/jesp.2024.373531
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Authors
SARAH IBRAHIM MOHAMED EZZ EL DINE1; ABEER SAID ABD EL-GHANY AL-ANTABLY1; MOHAMED M. EL BAHY2; DINA MOHAMED HAMDY EL AKAD1; ENAS M. A. RIZK1
1Department of Medical Parasitology, Kasr Al-Ainy Faculty of Medicine, Cairo, Egypt
2Department of Parasitology, Faculty of Veterinary Medicine, Cairo University, Egypt
Abstract
Blastocystis is an anaerobic parasite that was identified as the most common eukaryotic organism
reported in human fecal samples. Data on treatment options signifies that efficacious antimicrobial
eradication of Blastocystis spp. is still far from straight forward. As well as Metronidazole, has
shown some resistance. The study evaluated chitosan and chitosan Nano particles against Blastocystis
in vitro. Blastocystis species in stool samples were cultured. Culture tubes were divided into
three groups: Chitosan, Chitosan Nano particles and Metronidazole (reference drug), and positive
control. Different criteria were used to evaluate the tested drugs on Blastocystis and assessing
the viability before and after treatment by the use of Neutral red stain and recording structural
changes. Also, TEMand the single cell gel electrophoresis (SCGE)/comet assay were used.
The results showed a direct relation between increase in the concentration of Chitosan and Chitosan
nano-particles and exposure time and increase in the mortality rates. TEM showed morphological
damage when exposed to tested drug, Comet assay showed direct relationship between the
increase in the dose of Chitosan Nano particles NPs and the degree of DNA genotoxic destruction.
Keywords
Blastocystis species; Chitosan; Chitosan nanoparticles; electron microscope
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