Phenotypic Detection of Some Beta-lactamases and Enterobacterial Repetitive Intergenic Consensus Genotyping (ERIC) of Natively Isolated Uropathogenic Escherichia coli | ||||
| Egyptian Journal of Medical Microbiology | ||||
| Article 10, Volume 33, Issue 4, October 2024, Page 75-82 PDF (650.5 K) | ||||
| Document Type: New and original researches in the field of Microbiology. | ||||
| DOI: 10.21608/ejmm.2024.307213.1289 | ||||
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| Authors | ||||
Ebtehal E. Edrees  ; Ahmed A. Hussein; Noor A. Al-Buhamrah
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| Department of Pathological Analyses, Faculty of Science, University of Kufa, Najaf, Iraq | ||||
| Abstract | ||||
| Background: β-lactamases comprise wide number of enzymes sharing a common goal which is the hydrolysis of β-lactam anibiotics. Enterobacterial Repetitive Intergenic Consensus –Polymerase Chain Reaction is considered as one of the most reliable fingerprinting technique that provides sharp and fast results with relatively low cost. Objective: The current study aimed to isolate Uropathogenic E. coli and explore the possession of some β-lactamases phenotypically and to genetically typing the β-lactam resistant isolates. Methodology: During the study period between November 2023 and January 2024, (80) urine samples were collected from (66 )female and (14 )male patients , who visited hospitals in Al- Najaf Al-Ashraf province in order to isolate uropathogenic Escherichia coli. Identification was done using cultural and biochemical tests supported by utilizing Vitek-2 system. Antibiotic sensitivity test was used to determine pattern of resistance, Screening tests of β-lactamases comprised (DDST) and (CST) on those showed resistance to ceftriaxone. Genetic study included the use of Enterobacterial Repetitive Intergenic Consensus –Polymerase Chain Reaction (ERIC-PCR) technique to determine genetic variation of those isolates. Results: In this study (26) isolates were obtained. AST results showed that total number of isolates were resistant to tetracycline, while (84.6%) were resistant to trimethoprim, (53.8%) were resistant to ceftriaxone. While resistant and sensitive isolates were equal regarding ciprofloxacin.. Results showed that out of 14 cefitriaxone resistant isolates three (21.4%) of isolates were resistant to imipenem. Screening method of (MBLs) showed that threes isolates (EC3, EC8, and EC14) gave positive results (MBL producers) for both tests. Regarding the screening the Extended spectrum beta lactamases, results were negative where the isolates were either susceptible to β-lacatam antibiotics involved in the test, or resistant to all antibiotics used. Furthermore, genetic analysis of (ERIC-PCR) revealed at similarity ≥ 70% the presence of 9 different clusters, eight of them with single isolate, other three clusters with two identical pattern each. Identical pattern was shown in two of MBL producers (EC3, EC14). Conclusion: We conclude that native Escherichia coli isolates had the ability to produce metallo beta-lactamases, they produced various ERIC patterns, which reflects the high genetic variation these bacteria possess, though they caused the same infection (urinary tract infection), and presence of convergence may reflect phenotypic similarity of resistance pattern. | ||||
| Keywords | ||||
| Beta-lactamases; MBL; ERIC –PCR; Escherichia coli; UTI | ||||
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