Isolation and Identification of Methicillin-Resistant Staphylococcus aureus (MRSA) From Different Sources of Food in Basra Province / Iraq | ||||
| Egyptian Journal of Medical Microbiology | ||||
| Article 12, Volume 33, Issue 4, October 2024, Page 91-97 PDF (411.66 K) | ||||
| Document Type: New and original researches in the field of Microbiology. | ||||
| DOI: 10.21608/ejmm.2024.311434.1303 | ||||
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| Authors | ||||
Salah N. Aziz  1; Khulood A. Huseein2; Ameer A. Mohammed3; Teif A. Najm4
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| 1Department of Food Science, Agriculture College, University of Basrah, Basrah, Iraq | ||||
| 2Department of Medical Science, Nursing College, University of Basrah, Basrah, Iraq | ||||
| 3Department of Marine Vertebrate, Marine Science Center, University of Basrah, Basrah, Iraq | ||||
| 4Department of Biology, College of Education of Al Qurna, University of Basrah, Basrah, Ira | ||||
| Abstract | ||||
| Background: Staphylococcal food-borne illness (SFD) is a food-borne infection that is highly common around the world. MRSA is a major cause of infections and poses a significant global health threat due to the toxins it can produce in food. Objective: is to characterize S. aureus isolates from foodstuffs collected from Basrah. Methodology: A total of 135 food samples of locally made food products were collected from different areas of Basrah markets in February 2022. The samples included (milk, cake, cheese), and various types of meats (beef, chicken, lamb). Results and Discussion: showed the presence 27 strains of S. aureus in percentage of 20% of the diverse samples (135 samples). 10.7% of the meat samples, 6.2% of the milk samples, 1% of the cake samples, and 2.1% of the cheese samples all had S. aureus. Using the Polymerase Chain Reaction (PCR), a total of S. aureus isolates from different food products were collected and identified. The detection of the mecA gene identified MRSA isolates. PCR amplification of the 27 phenotypically S. aureus isolates revealed that 4 were found to carry mecA genes in a percentage of (14.81%), exhibited resistance to methicillin, and were identified as (MRSA). The following amplicon sizes were revealed by the gel electrophoresis results: (DNA fragments) 756 bp, 500 bp (16S rDNA gene of S. aureus), and 310 bp (mecA gene). Conclusion: our results suggest that artisanal food producers should have better control production hygiene measure because it represents a potential risk to public health. | ||||
| Keywords | ||||
| SFD; MRSA; mecA genes; Staphylococcus aureus | ||||
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