EFFECT OF THIOUREA ON MOTILITY AND VIABILITY OF CHILLED-STORED AND FROZEN-THAWED GOAT SPERMATOZOA | ||||
| Veterinary Medical Journal (Giza) | ||||
| Volume 51, Issue 1, January 2003, Page 49-57 PDF (2.31 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/vmjg.2003.375134 | ||||
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| Authors | ||||
| M WAHEED* ; T KHALIFA; K EL-SHAHAT | ||||
| Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza 12515, Egypt. | ||||
| Abstract | ||||
| The aim of the present study was to elucidate the effect of thiourea on motility and viability of chilled-stored and frozen-thawed goat spermatozoa. Five male Baladi goats were used in three experiments during this work. Three different Concentrations (10,50 and 100 mg %) of thiourea were added to semen extenders before processing. Tris-based egg yolk and Tris-egg yolk free extenders were used for chilled-storage of spermatozoa in the first and second experiments, respectively. In the third experiment, motility and viability of frozen-thawed goat semen were evaluated after inclusion of 50 mg% thiourea in the extender. In vitro augmentation of semen extender with 50 mg % thiourea exerted a pronounced improvement in incubated and post-thawed sperm motility percentages and viability indices of goat spermatozoa either in presence or absence of egg yolk. It is concluded that Tris-egg yolk free extenders could be used in presence of 50 mg % thiourea for both incubation and preservation goat semen. | ||||
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