DIRECT DETECTION OF VARIANT INFECTIOUS BURSAL DISEASE VIRUS IN VACCINATED EGYPTIAN BROILER FLOCKS USING ANTIGEN-CAPTURE ELISA
METWALLY, A., SABRY, M., SAMY, A., OMAR, M., YOUSIF, A., REDA, I. (2003). DIRECT DETECTION OF VARIANT INFECTIOUS BURSAL DISEASE VIRUS IN VACCINATED EGYPTIAN BROILER FLOCKS USING ANTIGEN-CAPTURE ELISA. EKB Journal Management System, 51(1), 105-119. doi: 10.21608/vmjg.2003.375142
A METWALLY; M SABRY; A SAMY; M OMAR; A YOUSIF; I REDA. "DIRECT DETECTION OF VARIANT INFECTIOUS BURSAL DISEASE VIRUS IN VACCINATED EGYPTIAN BROILER FLOCKS USING ANTIGEN-CAPTURE ELISA". EKB Journal Management System, 51, 1, 2003, 105-119. doi: 10.21608/vmjg.2003.375142
METWALLY, A., SABRY, M., SAMY, A., OMAR, M., YOUSIF, A., REDA, I. (2003). 'DIRECT DETECTION OF VARIANT INFECTIOUS BURSAL DISEASE VIRUS IN VACCINATED EGYPTIAN BROILER FLOCKS USING ANTIGEN-CAPTURE ELISA', EKB Journal Management System, 51(1), pp. 105-119. doi: 10.21608/vmjg.2003.375142
METWALLY, A., SABRY, M., SAMY, A., OMAR, M., YOUSIF, A., REDA, I. DIRECT DETECTION OF VARIANT INFECTIOUS BURSAL DISEASE VIRUS IN VACCINATED EGYPTIAN BROILER FLOCKS USING ANTIGEN-CAPTURE ELISA. EKB Journal Management System, 2003; 51(1): 105-119. doi: 10.21608/vmjg.2003.375142

DIRECT DETECTION OF VARIANT INFECTIOUS BURSAL DISEASE VIRUS IN VACCINATED EGYPTIAN BROILER FLOCKS USING ANTIGEN-CAPTURE ELISA

Veterinary Medical Journal (Giza)
Volume 51, Issue 1, January 2003, Page 105-119  XML PDF (3.2 MB)
Document Type: Original Article
DOI: 10.21608/vmjg.2003.375142
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Authors
A METWALLY* 1; M SABRY2; A SAMY3; M OMAR1; A YOUSIF3; I REDA3
1Private Poultry Diagnostic Laboratory, Giza, Egypt.
2Egytech, Dokki, Cairo, Egypt.
3Virology Department, Faculty of Veterinary Medicine, Cairo University, Egypt.
Abstract
Infectious bursal disease virus (IBDV) serotype I isolates result in serious economic losses to the Worldwide poultry industry. Variant IBDV strains Are usually isolated from flocks vaccinated using classical IBDV vaccines. Monoclonal antibody typing has been used to differentiate between classical and variant strains. A total of 277 bursal samples from 13 vaccinated broiler flocks were Tested for IBDV subtypes. A panel of 4 monoclonal antibodies directed against VP2 epitopes was used to test the bursal homogenates using an antigen-capture ELISA (AC-ELISA). Of the total Number of samples, 73.6% were IBDV-antigen- Positive, of which, 41.7% were classical IBDV And 58.3% were variant IBDV. Del/E was the predominant variant detected in the tested flocks (51.3% of IBDV-positive samples). Moreover, 47% of the total variants were untypeable. The Presence of variant IBDV in vaccinated Egyptian Broiler flocks demonstrates the need for a revision of current control strategies and vaccination Programs.
Keywords
IBDV; Classical; Variant; Antigen-Capture; Antigenic profile
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