In Vitro Assessment of Antimicrobial Activity of Chitosan Nanoparticles Loaded with the Honeybee, Apis mellifera Venom
Hassan, M., El-dek, S., Mohamed, A., Abdelwahab, A. (2019). In Vitro Assessment of Antimicrobial Activity of Chitosan Nanoparticles Loaded with the Honeybee, Apis mellifera Venom. EKB Journal Management System, 12(3), 85-103. doi: 10.21608/eajbsa.2019.37526
Mostafa I. Hassan; S. I. El-dek; Aly F. Mohamed; Abdelwahab Khalil Abdelwahab. "In Vitro Assessment of Antimicrobial Activity of Chitosan Nanoparticles Loaded with the Honeybee, Apis mellifera Venom". EKB Journal Management System, 12, 3, 2019, 85-103. doi: 10.21608/eajbsa.2019.37526
Hassan, M., El-dek, S., Mohamed, A., Abdelwahab, A. (2019). 'In Vitro Assessment of Antimicrobial Activity of Chitosan Nanoparticles Loaded with the Honeybee, Apis mellifera Venom', EKB Journal Management System, 12(3), pp. 85-103. doi: 10.21608/eajbsa.2019.37526
Hassan, M., El-dek, S., Mohamed, A., Abdelwahab, A. In Vitro Assessment of Antimicrobial Activity of Chitosan Nanoparticles Loaded with the Honeybee, Apis mellifera Venom. EKB Journal Management System, 2019; 12(3): 85-103. doi: 10.21608/eajbsa.2019.37526

In Vitro Assessment of Antimicrobial Activity of Chitosan Nanoparticles Loaded with the Honeybee, Apis mellifera Venom

Egyptian Academic Journal of Biological Sciences. A, Entomology
Article 8, Volume 12, Issue 3, June 2019, Page 85-103  XML PDF (1.5 MB)
Document Type: Original Article
DOI: 10.21608/eajbsa.2019.37526
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Authors
Mostafa I. Hassan1; S. I. El-dek2; Aly F. Mohamed3; Abdelwahab Khalil Abdelwahab4
1.Department of Zoology and Entomology, Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt
2Materials Science and Nanotechnology Department, Faculty of Postgraduate Studies for Advanced Sciences (PSAS), Beni-Suef University, Beni-Suef 62511, Egypt
33Applied Researches Sector, Vacsera, Giza, Egypt
4Zoology Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt
Abstract
         The aim of the present study was to extract chitosan from corpses of the naturally died honeybee, prepare chitosan nanoparticles (B- CS- NPs) for loading the honeybee, Apis mellifera venom (BV) and evaluate their antimicrobial potential. Chitin was extracted from the cuticle of corpses of naturally died honeybees following 4 steps; de-waxing, demineralization, deproteinization and discoloration. Chitosan was obtained by deacetylation of chitin and characterized using the Fourier transform infrared (FTIR) and X-ray diffraction. Honeybee chitosan nanoparticles (B-CS-NPs) were prepared by ionic gelation method using TPP in acidic medium. Empty nanoparticles (B-CS-NPs) and bee venom loaded nanoparticles (BV loaded NPs) were characterized. Hydrodynamic size and zeta potential of B- CS NPs were 74.2 nm, and 51.1 mV, while those of bee venom-loaded (BV loaded NPs) were 110.5 nm, 49.0 mv, respectively. The loading capacity (LC) and encapsulation efficiency (EE) were 86.5 % and 91.3 %, respectively, at bee venom concentration of 600 µg / ml. The antimicrobial activity of empty and BV loaded nanoparticles was studied using different strains of human pathogenic bacteria and fungi. Compared to empty nanoparticles, BV loaded NPs exhibited potent antimicrobial activity against the studied strains except in Aspergillus flavus fungus, which seemed to be resistant.
Keywords
Nanoparticles; Honeybees; Bee venom
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