The Combined Effect of ACE, TCF7L2, and PPARGC1A Gene Polymorphisms in Diabetic Nephropathy
Gouda, W., Ismail, M., Shaker, O., Wahba, E., Yousif, H., Afify, M. (2017). The Combined Effect of ACE, TCF7L2, and PPARGC1A Gene Polymorphisms in Diabetic Nephropathy. EKB Journal Management System, 60(5), 869-881. doi: 10.21608/ejchem.2017.1335.1084
Weaam Gouda; Manal Fouad Ismail; Olfat Gamil Shaker; Esmat Wahba; Heba Mourad Yousif; Mie Afify. "The Combined Effect of ACE, TCF7L2, and PPARGC1A Gene Polymorphisms in Diabetic Nephropathy". EKB Journal Management System, 60, 5, 2017, 869-881. doi: 10.21608/ejchem.2017.1335.1084
Gouda, W., Ismail, M., Shaker, O., Wahba, E., Yousif, H., Afify, M. (2017). 'The Combined Effect of ACE, TCF7L2, and PPARGC1A Gene Polymorphisms in Diabetic Nephropathy', EKB Journal Management System, 60(5), pp. 869-881. doi: 10.21608/ejchem.2017.1335.1084
Gouda, W., Ismail, M., Shaker, O., Wahba, E., Yousif, H., Afify, M. The Combined Effect of ACE, TCF7L2, and PPARGC1A Gene Polymorphisms in Diabetic Nephropathy. EKB Journal Management System, 2017; 60(5): 869-881. doi: 10.21608/ejchem.2017.1335.1084

The Combined Effect of ACE, TCF7L2, and PPARGC1A Gene Polymorphisms in Diabetic Nephropathy

Egyptian Journal of Chemistry
Article 12, Volume 60, Issue 5, October 2017, Page 869-881  XML PDF (1.25 MB)
DOI: 10.21608/ejchem.2017.1335.1084
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Authors
Weaam Gouda email orcid 1; Manal Fouad Ismail2; Olfat Gamil Shaker3; Esmat Wahbaorcid 1; Heba Mourad Yousif4; Mie Afify1
1Biochemistry Department, Genetic engineering and biotechnology division, National Research Center, Egypt.
2Biochemistry Department, Faculty of Pharmacy, Cairo University
3Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University
4Internal Medicine, Faculty of Medicine, Cairo University
Abstract
Objective: This study was performed for investigation the relationship between variants of ACE, TCF7L2 and PPARGC1A gene polymorphisms individually or in combination with the development of nephropathy in T2DM.
Subject and Methods: The study was included 85 T2DM patients (45 with nephropathy and 40 without nephropathy), and 45 healthy control subjects. The I/D polymorphism of ACE gene was evaluated by PCR method. The polymorphisms rs7903146 (C/T) of TCF7L2 gene and Gly482Ser (G/A) and Thr394Thr (G/A) of PPARGC1A gene were evaluated by PCR-RFLP analysis.
Results: The frequency of ACE DD genotype and D allele was significantly higher in DN patients when compared to diabetic without nephropathy. The frequency of TCF7L2 rs7903146 TT genotype and T allele were significantly associated with DN patients compared to T2DM. Moreover, a significant association in A allelic frequencies was observed in DN cases compared to T2DM patients without nephropathy. No differences in the genotypic and allelic frequencies between T2DM patients with and without nephropathy were found for the Thr394Thr polymorphism.
Conclusions: Our study suggested that candidate gene polymorphisms I/D of ACE, rs7903146 of TCF7L2 and Gly482Ser of PPARGC1A individually or in combination may act as susceptibility biomarkers for nephropathy in T2DM.
Keywords
Diabetic nephropathy (DN); Type 2 diabetes mellitus (T2DM); Angiotensin-converting enzyme (ACE); Transcription factor 7–like 2 (TCF7L2); Peroxisome proliferator activated receptor gamma coactivator-1 alpha (PPARGC1A)
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