BIOLOGICAL DETECTION OF CLOSTRIDIUM PERFRINGENS ENTEROTOXIN ORIGINATED FROM CAMEL (CAMELUS DROMEDARIUS) ENTEROTOXAEMIA | ||||
| Veterinary Medical Journal (Giza) | ||||
| Volume 48, Issue 1, January 2000, Page 73-82 PDF (3.58 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/vmjg.2000.375598 | ||||
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| Author | ||||
| Y EL-NAENAEEY | ||||
| Department of Microbiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt | ||||
| Abstract | ||||
| An outbreak of enterotoxacmia in breeding cam-cls in Belbais arca was reported. A total of 68 or-gan samples (liver, kidney, spleen, heart, small in-testine, colon, lymph node, abomasum and rumen) collected from 10 dead animals were clin-icaly and batériologicaly examined. Of 40 bacteri-al isolates, 23 (57.5%) were identified as Clostrid- ium perfringens type A, 8 (20%) as Bacillus cercus and 9 (22.5%) as other Bacillus spp. Thebiological detection of C. perfringens enterotoxin was done in rabbits ileal loops. Rabbits ileal loops challenged with cell extract or culture filtrates of various strains actively responded by fluid accu-mulation and dilatation. The enterotoxin was shown to be heat labile and lost ils activity at pF| and 12. It was inactivated by pronase but not by amylase, lipase or trypsin enzymes. | ||||
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