IMMUNE RESPONSES OF IMMUNIZAED BALB/C MICE WITH AVIAN REO VIRUS | ||||
| Veterinary Medical Journal (Giza) | ||||
| Volume 44, Issue 1, January 1996, Page 41-47 PDF (5.97 MB) | ||||
| DOI: 10.21608/vmjg.1996.376416 | ||||
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| Authors | ||||
| AMINA .* 1; A. NAWWAR2; TH .2; ABDEL AZIZ .2; EL-EBIARY .2; A. .2; NADA .2; ILS .2 | ||||
| 1Animal Health Research Institute, Dokki, Cairo, Egypt | ||||
| 2Animal Health Research Institute, Dokki, Cairo, Egypt. | ||||
| Abstract | ||||
| As preliminary procedure for production of monoclonal antibodies against avian reovirus, cellular and humoral immunity were measured for immunized female balb/c mice after continuous exposure to purified avian reovirus 17.33 . Immunoglobulin concentrations of IgG, IgG1, IgG2a, IgG2b, IgG3, IgM, IgA, IgA were measured in sera of mice at different intervals post inoculation with one, two, three , four doses of purified avian reovirus 1733, using Radial immunodiffusion test. At the time antibody titers were measured in sera of immunized mice by enzyme immunosorbent assay using single dilution of sera. Immunoglobulin secreting cells were counted in spleen of immunized mice, one week post second, third and fourth doses of avian reovirus using solid phase immunoenzymatic technique. The results indicated that there was no relation between hyperimmunization of mice and optimum time to obtain mature, large differentiated specific-B-lymphocyte. | ||||
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