Histological Study on Possible Regenerative Effect of Bone Marrow Derived Mesenchymal Stem Cells versus Platelet Rich Plasma in Skeletal Muscle Injury Model in Adult Male Albino Rat | ||
| Egyptian Journal of Histology | ||
| Article 16, Volume 48, Issue 3, September 2025, Pages 1030-1045 PDF (3.05 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejh.2024.309725.2118 | ||
| Authors | ||
| Asmaa Mohamed Abdel Hameed1; Dalia Elmarakby2; Nada Abd Al-Wahab* 3; marwa sabry4 | ||
| 1Histology departement, faculty of medicine, cairo university, egypt | ||
| 2Histology department, Faculty of Medicine, Cairo University, Cairo, Egypt | ||
| 3histology department,faculy of medicine,cairo university,egypt | ||
| 4histology department,faculty of medicine,cairo universty,egypt | ||
| Abstract | ||
| Introduction and Aim of Work: Skeletal muscle injuries are one of the most common injuries.As the complete recovery is compromised due to fibrosis. This study was designed to evaluate and compare the possible regenerative effects of bone marrow-mesenchymal stem cells (BM-MSCs) versus platelet rich plasma (PRP) in skeletal muscle laceration injury in adult male albino rat model. Materials and Methods: Forty four adult rats were divided into 4 groups: Group I (Control)=12 rats, Group II=12 rats subjected to right gastrocnemius muscle laceration and subdivided into subgroup IIA=4 rats sacrificed at day0, subgroup IIB (recovery)=4 rats sacrificed at day 7, subgroup IIC (recovery)=4 rats sacrificed at day 14. Group III=10 rats (local IM injection of 1ml PBS containing 1 million BM-MSCs) subdivided into subgroup IIIA=4 rats sacrificed at day 7, subgroup IIIB=4 rats sacrificed at day14. Group IV=8 rats (local IM injection of 1 ml of PRP) subdivided into Subgroup IVA =4 rats sacrificed at day 7, Subgroup IVB =4 rats sacrificed at day 14. Samples were obtained and processed for biochemical, histological, and immunohistochemical techniques. Results: H&E, subgroup IIA confirmed the injury. Subgroup IIB showed irregular muscle fibers, inflammatory, fat cells infiltration and few fibers showed myotubes. Subgroup IIC showed some regular and many irregular muscle fibers with extensive apparent fibrosis. Groups III and IV showed improvement of muscular structure. Mallory stain, group IIA showed disturbed endomysium. Subgroups IIB&IIC showed significant increase in Collagen fibers deposition which significantly decreased in groups III&IV.As regard myogenin and VEGF reaction, treated groups were significantly higher than recovery groups, while group III was the highest .CPK value in subgroup IIA was significantly the highest with significant drop in recovery groups with more drop in treated groups. Conclusion: MSCs and PRP provided evidence for their therapeutic effects in muscle injury. Meanwhile, MSCs have the upper hand in restoring muscle structure. | ||
| Keywords | ||
| BM-MSCs; laceration; PRP; skeletal muscle | ||
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