ISOLATION AND PREPARATION OF BOVINE ROTA VIRUS (BRV) VACCINE IN MINNESOTA, USA
WASSEL, M. (1996). ISOLATION AND PREPARATION OF BOVINE ROTA VIRUS (BRV) VACCINE IN MINNESOTA, USA. EKB Journal Management System, 44(3), 583-591. doi: 10.21608/vmjg.1996.376625
M. WASSEL. "ISOLATION AND PREPARATION OF BOVINE ROTA VIRUS (BRV) VACCINE IN MINNESOTA, USA". EKB Journal Management System, 44, 3, 1996, 583-591. doi: 10.21608/vmjg.1996.376625
WASSEL, M. (1996). 'ISOLATION AND PREPARATION OF BOVINE ROTA VIRUS (BRV) VACCINE IN MINNESOTA, USA', EKB Journal Management System, 44(3), pp. 583-591. doi: 10.21608/vmjg.1996.376625
WASSEL, M. ISOLATION AND PREPARATION OF BOVINE ROTA VIRUS (BRV) VACCINE IN MINNESOTA, USA. EKB Journal Management System, 1996; 44(3): 583-591. doi: 10.21608/vmjg.1996.376625

ISOLATION AND PREPARATION OF BOVINE ROTA VIRUS (BRV) VACCINE IN MINNESOTA, USA

Veterinary Medical Journal (Giza)
Volume 44, Issue 3, July 1996, Page 583-591  XML PDF (5.28 MB)
Document Type: Original Article
DOI: 10.21608/vmjg.1996.376625
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Author
M. WASSEL*
Veterinary Serum and Vaccine Research Institute, Cairo,Egypt.
Abstract
Nine cytopathic bovine rota virus stratins were isolted in MA104 cells from fecal specimens of calves. Plaque assay was used for selection, titration and purification of small and large plaques of selected rota virus strains.
 Electron microscopy was used to detect the presence of particles with rota virus morphology in MA104 cells. Virus neutralization test was used for further identification of strains of high immunogenic response.
 
Pooling of these isolates and their use in making a formalized-inactivated bovine rota vaccine was tried.
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